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Cryopreservation of human semen in test yolk buffer medium or synthetic medium supplemented with phospholipid and antioxidant: a controlled clinical trial

Grant number: 14/13477-2
Support type:Scholarships in Brazil - Master
Effective date (Start): October 01, 2014
Effective date (End): September 30, 2016
Field of knowledge:Health Sciences - Medicine
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal researcher:Rosana Maria dos Reis
Grantee:Aline Bomfim Silva
Home Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil


The therapeutic semen cryopreservation, elective or for medical procedures such as radiotherapy, chemotherapy or invasive surgery, and also in assisted reproduction treatments, is of paramount importance. However, the conventional cryopreservation methods induce damage to sperm, associated with loss of motility and increase the sperm chromatin fragmentation. The conventional extenders medium based on egg yolk (test yolk buffer) contain additives of animal origin, which sets as less secure in terms of biosecurity, and its mechanism of action is not fully understood. In this respect, the new formulation of synthetic cryoprotective proposed in this project can, potentially, contribute to obtain accurate information about the action of cryoprotectants on the sperm cell and the development of safer strategies for semen cryopreservation. The objective of this project is to compare two media for cryopreservation of human semen, the conventional and synthetic supplemented with phospholipid and antioxidant, for the progressive sperm motility and sperm DNA fragmentation. In parallel, it will be evaluated the interaction of lipids present in cryoprotectant medium with the plasma membrane of the sperm. A clinical trial will be conducted, with randomized allocation of semen from the same patient for two parallel groups: control (ANTIOX-PC) and study (test yolk buffer). Quantitative parameters (semen analysis) and qualitative (TUNEL) of semen samples will be evaluated, before and after cryopreservation, in 63 volunteer patients recruited in the Infertility Clinic of the Division of Human Reproduction of Department of Gynecology and Obstetrics of HC-FMRP-USP. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SICCHIERI, FERNANDA; SILVA, ALINE BOMFIM; SANTANA, VIVIANE PAIVA; CARNEIRO VASCONCELOS, MARIA APARECIDA; FERRIANI, RUI ALBERTO; VIREQUE, ALESSANDRA APARECIDA; DOS REIS, ROSANA MARIA. Phosphatidylcholine and L-acetyl-carnitine-based freezing medium can replace egg yolk and preserves human sperm function. TRANSLATIONAL ANDROLOGY AND UROLOGY, v. 10, n. 1 JAN 2021. Web of Science Citations: 0.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
SILVA, Aline Bomfim. Cryopreservation of human semen in Yolk Buffer test medium or synthetic medium supplemented with phospholipid and antioxidant: controlled clinical trial. 2017. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Medicina de Ribeirão Preto Ribeirão Preto.

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