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| Author(s): |
Eduarda Antunes Moreira
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Ribeirão Preto. |
| Institution: | Universidade de São Paulo (USP). Faculdade de Ciências Farmacêuticas de Ribeirão Preto (PCARP/BC) |
| Defense date: | 2017-10-02 |
| Examining board members: |
Norberto Peporine Lopes;
Paulo Cezar Vieira
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| Advisor: | Norberto Peporine Lopes |
| Abstract | |
Considering that diverse chlorogenic acids\' biological activities have been described, a better understanding of their biosynthesis and accumulation is closely related to phytotherapics\' quality parameters. This research aimed to monitor the variation on the concentration of secondary metabolites from the chlorogenic acids\' group on the leaf\'s tissue of diverse species, towards different post-harvest treatments. The monitoring was performed through the quantification of the compounds by UPLCMS/ MS. On the first phase of the project, leaves from each selected species (Cashew - Anacardium occidentale L.; Soursop - Annona muricata L.; Pata-de-Vaca - Bauhinia variegata L.; Lemon - Citrus limon (L.) Burm. f.; Coffee - Coffea arabica L.; Cherry - Eugenia uniflora L.; Rosemary - Rosmarinus officinalis L., and Jambolan - Syzygium cumini (L.) Skeels) were harvested. This material was submitted to an extraction process, and the obtained extracts were analyzed by HPLC-MS/MS. Nine substances were found, monossubstituted on positions 3-, 4- and 5-, belonging to the subgroups of p-coummaroylquinic, caffeoylquinic and feruloylquinic acids, in addition to dicaffeoylquinic acids. The chromatographic signals\' identification was preceded observing the fragmentation pattern of each compound. On the second phase of the study, four leaves were collected from each individual. The leaves were frozen with liquid nitrogen (to interrupt the metabolism) on different moments - immediately after the collection (T0), 30 min after de collection (T0,5), 1 h after the collection (T1) and 2 h after the collection (T2). This procedure happened once a month during six months. On August, 2016, a different collection was performed for a broader observation: six leaves were harvested from three individuals from selected species (Rosemary, Cherry, Pata-de-Vaca, Limon and Coffee), and each leave was frozen on T0 (immediately after the collection), T6 (6 h after the collection), T12 (12 h after the collection), T24 (24 h after the collection), T48 (48 h after the collection) and Ts (after leaf\'s drying - approximately 30 days after collection). The harvested material was extracted and analyzed by UPLC-MS/MS, on MRM mode. The analytical method was validated for linearity, accuracy and precision. Chlorogenic acids\' concentrations did not show a pattern of variation that related to the different treatments or a linear increase on response until 2 hours after the beginning of the tissue catabolism. The samples submitted to a broader treatment interval, however, showed a significant rise on the concentration of different compounds, on leaves that did not have their metabolism interrupted until 30 days after the collection. This accumulation of chlorogenic acids agrees with the hypothesis that the raise on the concentration of these substances can be related to the catabolism of polymeric phenols of higher mass. (AU) | |
| FAPESP's process: | 15/09227-3 - Analysis of the metabolism variation of chlorogenic acid and its derivatives |
| Grantee: | Eduarda Antunes Moreira |
| Support Opportunities: | Scholarships in Brazil - Master |