Advanced search
Start date

Chlorogenic acid and its relation with lignin biosynthesis

Grant number: 14/17831-5
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): December 01, 2014
Effective date (End): February 15, 2019
Field of knowledge:Agronomical Sciences - Agronomy
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal researcher:Paulo Mazzafera
Grantee:Nathalia Volpi e Silva
Home Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Associated scholarship(s):16/15834-2 - CRISPR/Cas9 genome editing to modify lignin biosynthesis in Nicotiana tabacum, BE.EP.DR


Phenylpropanoids are compounds derived from phenylalanine and they are involved in various aspects related to biotic and abiotic stress defense. One of phenylpropanoids present in most plants, and in some of which in considerable amount is chlorogenic acid (CGA). CGA biosynthesis is mediated by the enzyme quinate hydroxycinnamoyl transferase (HQT). Although it has not been proven yet, some studies have been suggesting that the CGAs pool could be related to the lignin biosynthesis in plants. Another enzyme, shikimate hydroxycinnamoyl transferase (HCT) appears to be involved both in the lignin biosynthetic pathway and in the CGAs route. As the HCT, HQT uses the p-coumaril CoA to form hydroxycinnamoyl shikimate or quinate hydroxycinnamoyl esters respectively. Furthermore, caffeoyl esterase enzyme shikimate (CSE) has been recently described as involved in the conversion of shikimate in caffeoyl caffeate, which is subsequently converted to caffeoyl CoA, in lignin pathway. Thus, the CSE shares the substrate with the enzyme HCT, suggesting that an alteration in its expression may interfere not only in the metabolism of lignin, but also in the CGA metabolism one. Due to the fact that they have common intermediates, it is possible to have interdependence between the synthesis in CGA and lignin pathways, and that CGA may act as a donor of carbon skeletons for the lignin biosynthesis. Thus, the construction of mutants and double mutants over expressing and silencing HCT, HQT and CSE genes may help elucidate the nature of this interdependence among the CGA and lignin pool as well as validate the role of the enzyme CSE as a common component in the lignin pathway. It is expected that with phenotypic, biochemical and molecular analyzes information confirming that CGA may be redirected for the synthesis of lignin and, not only HCT, but also HQT and CSE enzymes, would have a role in both pathways. (AU)

News published in Agência FAPESP Newsletter about the scholarship:
Articles published in other media outlets (0 total):
More itemsLess items

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
VOLPI E SILVA, NATHALIA; MAZZAFERA, PAULO; CESARINO, IGOR. Should I stay or should I go: are chlorogenic acids mobilized towards lignin biosynthesis?. Phytochemistry, v. 166, OCT 2019. Web of Science Citations: 7.

Please report errors in scientific publications list by writing to: