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| Author(s): |
Kayo José Garcia de Almeida Castilho Neto
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Jaboticabal. 2018-10-16. |
| Institution: | Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal |
| Defense date: | 2018-08-31 |
| Advisor: | Fabiano Antonio Cadioli |
| Abstract | |
Trypanosomiasis is disease caused by agents of the Trypanosoma genus, which infect humans, domestic and wild animals on the African, Asian, South and Central Americans continents. The clinical signs of the disease, such as anemia and weight loss, are generic and also due to the presence of parasitic intervals, the diagnosis of the disease becomes challenging. Chemotherapics such as diminazine aceturate (AD) and isomethemidum hydrochloride (ISM) are available for the treatment of the disease in Brazil, although there are reports and indications of resistance to the principles. This work aimed to use loop-mediated isothermal amplification (LAMP) in the diagnosis and to evaluate the parasitological cure of cattle naturally infected by Trypanosoma vivax after treatment with ISM. Thirty bovines naturally infected with T. vivax were treated with two ISM applications at a dose of 1.0 mg / kg intramuscularly on days 0 and 150. Blood and serum samples were collected on 0, 7, 15, 30, 60, 90, 120, 150, 180, 210 and 240 days after treatment and were submitted to T. vivax diagnosis by polymerase chain reaction (PCR) targeting the CatL-Like, LAMP targeting the satellite DNA gene and enzyme immunoabsorption assay (ELISA) with soluble T. vivax antigen. Also, an additional PCR for diagnosis of T. theileri infection directed to the CATL gene was performed. There was persistence in the detection of T. vivax DNA in PCR and LAMP as well as continuous detection of anti-T. vivax antibodies by the ELISA method after the treatment, suggesting the presence of resistance of the parasite to the ISM. All samples tested for the diagnosis of T. theileri by PCR were negative. LAMP proved to be very useful in the diagnosis of naturally infected animals, but the combination of the LAMP and ELISA tests can avoid false diagnoses, contributing to a better control of the disease. The data indicate that PCR is not indicated for diagnosis in animals with low parasitemia, therefore is not recommended to promote the follow-up of animals after treatment with trypanocidal drugs. (AU) | |
| FAPESP's process: | 15/25397-6 - Lamp use for diagnosis and evaluation of therapeutic effectiveness of isometamidium chloride in bovine naturally infected with Trypanosoma vivax |
| Grantee: | Kayo José Garcia de Almeida Castilho Neto |
| Support Opportunities: | Scholarships in Brazil - Master |