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Cytokine profile determined by ELISA in cattle experimentally infected by Trypanosoma vivax

Grant number: 16/17401-6
Support type:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): January 09, 2017
Effective date (End): July 08, 2017
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
Principal Investigator:Fabiano Antonio Cadioli
Grantee:Otávio Luiz Fidelis Júnior
Supervisor abroad: Gene Wijffels
Home Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
Local de pesquisa : CSIRO Agriculture and Food, Australia  
Associated to the scholarship:14/10572-4 - Cytokines profile and its relation with parasitemia in cattle experimentally infected by Trypanosoma vivax, BP.DR

Abstract

Bovine herd outbreaks by Trypanosoma vivax have occurred more frequently in Brazil, although Brazilian Ministry of Agriculture, Livestock and Supply (MAPA) does not consider this disease a problem and because of this, there is neither official control nor accurate records about its occurrence. In the future, due to its spread, T. vivax can bring greater livestock losses due to deaths and reducing production rates, that are observed when introduced into herds on non-endemic areas. However, the disease diagnosis is a challenging task since many clinical, laboratory and immunological aspects are not fully elucidated. The aim of this study is to assess whether there is any variation of cytokines profile, IL-1b, IL-6, IL-10, TNFa and IFNg by ELISA in cattle experimentally infected with this hemoparasite; blood samples from three experimentally infected Girolando female (E1 to E3) with 2.0 x 107 trypomastigotes of "Lins" strain of T. vivax, obtained seven days before inoculation (D-7), on the day of inoculation (D0) , on the day following the inoculation (D1) and thereafter every seven days until 120 days after infection (DAI), will be used. The results obtained will help in understanding the pathophysiology of disease caused by T. vivax and may contribute to a refinement of disease diagnosis. (AU)