Advanced search
Start date
Betweenand

Hematological, biochemical, sorologic and histopathological changes of naturally and experimentally infected bovines by Trypanosoma vivax

Abstract

The aim of this project is detect hematological, biochemical, sorological and histopathological changes of naturally and experimentally infected Girolando cattle with Trypanosoma vivax. Eight adult female Girolando bovine will be used. Three naturally infected (N1 to N3); three experimentally infected (E1 to E3) with Tripanossoma vivax and two as control group (T1 and T2). The naturally infected animals were obtained during an outbreak in Lins (SP). Animals E1 to E3 will be inoculate IV with 2.0 x 107 T. vivax trypomastigotes. Blood samples will be obtain at each three days for hematological, parasitological and biochemical determinations. Serum samples will stored at -70°C until use. All samples will tested by IFAT and ELISA for anti-T. vivax antibodies. Eletrophoresis in SDS-PAGE for identification and quantification of acute phase proteins will perform. Animals will be euthanatized at 120 DPI or when show clinical and/or hematological life treating complications. Fragments from different tissues will collect and process for histological analysis. (AU)

Articles published in Agência FAPESP Newsletter about the research grant:
More itemsLess items
Articles published in other media outlets ( ):
More itemsLess items
VEICULO: TITULO (DATA)
VEICULO: TITULO (DATA)

Scientific publications (6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
FIDELIS JUNIOR, OTAVIO LUIZ; SAMPAIO, PAULO HENRIQUE; GONCALVES, LUIZ RICARDO; MACHADO, ROSANGELA ZACARIAS; ANDRE, MARCOS ROGERIO; WIJFFELS, GENE; CADIOLI, FABIANO ANTONIO. A Preliminary Study on the Relationship between Parasitaemia and Cytokine Expression of Peripheral Blood Cells in Trypanosoma vivax-Experimentally Infected Cattle. ANIMALS, v. 11, n. 11, . (16/17401-6, 12/02284-3, 14/10572-4)
FIDELIS JUNIOR, OTAVIO LUIZ; SAMPAIO, PAULO HENRIQUE; GONCALVES, LUIZ RICARDO; ANDRE, MARCOS ROGERIO; MACHADO, ROSANGELA ZACARIAS; WIJFFELS, GENE; CADIOLI, FABIANO ANTONIO. Comparison of conventional and molecular techniques for Trypanosoma vivax diagnosis in experimentally infected cattle. REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA, v. 28, n. 2, p. 7-pg., . (12/02284-3)
FIDELIS JUNIOR, OTAVIO LUIZ; SAMPAIO, PAULO HENRIQUE; MACHADO, ROSANGELA ZACARIAS; ANDRE, MARCOS ROGERIO; MARQUES, LUIZ CARLOS; CADIOLI, FABIANO ANTONIO. Evaluation of clinical signs, parasitemia, hematologic and biochemical changes in cattle experimentally infected with Trypanosoma vivax. REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA, v. 25, n. 1, p. 13-pg., . (11/15945-5, 12/02284-3)
FIDELIS JUNIOR, OTAVIO LUIZ; SAMPAIO, PAULO HENRIQUE; GONCALVES, LUIZ RICARDO; ANDRE, MARCOS ROGERIO; MACHADO, ROSANGELA ZACARIAS; WIJFFELS, GENE; CADIOLI, FABIANO ANTONIO. Comparação de técnicas convencionais e moleculares para diagnóstico em bovinos experimentalmente infectados por Trypanosoma vivax. Revista Brasileira de Parasitologia Veterinária, v. 28, n. 2, p. 203-209, . (12/02284-3)
FIDELIS JUNIOR, OTAVIO LUIZ; SAMPAIO, PAULO HENRIQUE; MACHADO, ROSANGELA ZACARIAS; ANDRE, MARCOS ROGERIO; MARQUES, LUIZ CARLOS; CADIOLI, FABIANO ANTONIO. Avaliação dos sinais clínicos, parasitemia e alterações hematológicas e bioquímicas de bovinos experimentalmente infectados peloTrypanosoma vivax. Revista Brasileira de Parasitologia Veterinária, v. 25, n. 1, p. 69-81, . (12/02284-3, 11/15945-5)
CADIOLI, FABIANO ANTONIO; FIDELIS JUNIOR, OTAVIO LUIZ; SAMPAIO, PAULO HENRIQUE; DOS SANTOS, GIULIANA NASCIMENTO; ANDRE, MARCOS ROGERIO; GARCIA DE ALMEIDA CASTILHO, KAYO JOSE; MACHADO, ROSANGELA ZACARIAS. Detection of Trypanosoma vivax using PCR and LAMP during aparasitemic periods. Veterinary Parasitology, v. 214, n. 1-2, p. 174-177, . (14/04066-9, 12/02284-3, 11/15945-5, 14/11600-1)