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| Author(s): |
Valéria Barbosa Souza
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | Campinas, SP. |
| Institution: | Universidade Estadual de Campinas (UNICAMP). Faculdade de Ciências Médicas |
| Defense date: | 2015-08-20 |
| Examining board members: |
André Almeida Schenka;
Patricia Sabino de Matos;
Paulo César Pires Rosa;
Helenice Gobbi;
Elaine Conceição de Oliveira
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| Advisor: | André Almeida Schenka |
| Abstract | |
Recently in a new paradigm of carcinogenesis, cancer stem cells (CSCs) have been regarded as responsible for the origin, morphological heterogeneity, self-renewal and therapeutic resistance of malignant neoplasms. As such, they have become an important target in pathophysiological/pharmacological studies of cancer. Currently, one of the main obstacles to the study of CSCs is the lack of reliable biomarkers. Although there seems to be some consensus regarding what should be considered typical CSC markers in vitro studies, there is very poor agreement when these are contrasted to in vivo studies based on human tumor tissue. Using three distinct breast cancer-derived cell lines, we investigated the expression markers in two biological contexts reflecting differences in microenvironmental (in vitro vs in vivo), and in two immunophenotyping techniques (flow cytometry vs immunocytochemistry). Our results show that the expression of most CSC markers is higher in vitro than in vivo, regardless of cell line. Furthermore, the expression of CSC markers is higher for immunocytochemistry than for flow cytometry. Finally, there have not been strong and significant correlations between most markers. Taken altogether, our data suggest: (1) that both the microenvironment and the immunodetection technique affect CSC marker expression, (2) that immunocytochemistry is probably the most sensitive technique for the detection of CSCs within solid tumors and (3) that the majority of CSC marker identifies distinct CSC subclone (AU) | |
| FAPESP's process: | 11/11609-0 - In vitro and in vivo characterization of immunomarkers for cancer stem cells in a human breast cancer cell line |
| Grantee: | Valéria Barbosa de Souza |
| Support Opportunities: | Scholarships in Brazil - Doctorate |