Full text
| |
| Author(s): |
Diego de Freitas Coêlho
Total Authors: 1
|
| Document type: | Doctoral Thesis |
| Press: | Campinas, SP. |
| Institution: | Universidade Estadual de Campinas (UNICAMP). Faculdade de Engenharia Química |
| Defense date: | 2016-07-14 |
| Examining board members: |
Elias Basile Tambourgi;
Ana Paula Brescancini Rabelo;
Juliana Ferrari Ferreira Secato;
Priscila Gava Mazzola;
Luiz Carlos Bertevello
|
| Advisor: | Edgar Silveira Campos; Elias Basile Tambourgi |
| Abstract | |
Bromelain is the generic name given to endopeptidases set coming from the members of the Bromeliaceae family, which owns the pineapple (Ananas comosus) and curauá (Pineapple erectifolius). Due to the important physiological roles they play, these enzymes provide a broad spectrum of proven therapeutic applications (anti-inflammatory action, anti-tumour, adjunct in wound healing, increase in cardiac capacity, etc.), and are used by the food industry in meat processing. The study bromelain extraction from organic residues (produced during the processing of pineapple), using aqueous two-phase systems (ATPS) offers a potential alternative to its purification and production. Additionally, annular centrifugal extractors are little studied equipment in Brazil, but that offer performance and efficiency higher than those obtained with conventional equipment. Thus, studies that aim to purify the bromelain from waste, using methods and processes, unusual and with the objective of marketing for the pharmaceutical industry are highly promising scientific and technological point of view. This work focuses on studying and development of a variant of the puller able to obtain bromelain by use of ATPS in the processing of waste referred to through the use of computational fluid dynamics (CFD) tools. Both the optimization of the ATPS as simulation process itself required the use of experimental design, as well as tools that aids have been developed throughout the project. As a result, it was possible to obtain bromelain with purity of commercial samples available and build a prototype extractor studied using widely known composite materials (AU) | |
| FAPESP's process: | 11/20733-7 - Development of Anular Centrifugal Contactor and its Application to Purification of Bromelain from Agroindustrial Waste through unconventional Aqueous Two-Phase Systems |
| Grantee: | Diego de Freitas Coêlho |
| Support Opportunities: | Scholarships in Brazil - Doctorate |