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Development of Anular Centrifugal Contactor and its Application to Purification of Bromelain from Agroindustrial Waste through unconventional Aqueous Two-Phase Systems

Grant number: 11/20733-7
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): March 01, 2012
Effective date (End): February 29, 2016
Field of knowledge:Engineering - Chemical Engineering - Industrial Operations and Equipment for Chemical Engineering
Principal researcher:Elias Basile Tambourgi
Grantee:Diego de Freitas Coêlho
Home Institution: Faculdade de Engenharia Química (FEQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Associated scholarship(s):13/11395-6 - Use of countercurrent chromatography applied on aqueous two-phase systems for purify bromelain from agroindustrial wastes, BE.EP.DR


Bromelain is the general name given to the group of endopeptidases found in pineapple (Ananas comosus) and curauá (Ananas erectifolius) as well as all proteases derived from members of the family Bromeliaceae. Due to its important physiological role, these enzymes offer a wide spectrum of proven therapeutic applications (anti-inflammatory, anticancer and fibrinolytic activities; reduction of healing time, increase of cardiorespiratory fitness, etc), besides being used for the meat processing in food industries. For industrial production of bromelain, the pineapple processing wastes should be preferentially used (as well as for the curauá), once in these there are large amounts of target molecule and its main products (i.e., fruit and juice from pineapple and fibers from curauá) have high commercial value.Therefore, the study of bromelain extraction from agroindustrial wastes, using aqueous two-phase systems (ATPS) can be an interesting alternative for its purification and production. Besides, water is the largest constituent in these systems, between 70% and 90%, and offers a pleasant enough environment, preserving the molecular stability of biomolecules. Additionally, annular centrifugal contactors are little studied in Brazil, but they offer superior performance and efficiency to those obtained in conventional equipment. Therefore, studies that aim to purify the bromelain from waste, with the use of methods and processes unconventional, with the objective of its commercialization for the pharmaceutical industry are highly promising in terms of science and technology.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
COELHO, DIEGO F.; SATURNINO, THAIS PERON; FERNANDES, FERNANDA FREITAS; MAZZOLA, PRISCILA GAVA; SILVEIRA, EDGAR; TAMBOURGI, ELIAS BASILE. Azocasein Substrate for Determination of Proteolytic Activity: Reexamining a Traditional Method Using Bromelain Samples. BIOMED RESEARCH INTERNATIONAL, . (11/20733-7, 12/14533-8)
COELHO, DIEGO F.; HUDDLESTON, JONATHAN G.; TUNDISI, LOUISE L.; MENEZES, PEDRO V. O.; PORTO, CARLA C. S.; MAZZOLA, PRISCILA G.; SOUZA, ROBERTO R.; TAMBOURGI, ELIAS B.. LLSR-An R Package for Data Analysis of Aqueous Two-Phase Systems. JOURNAL OF CHEMICAL AND ENGINEERING DATA, v. 64, n. 11, p. 4815-4826, . (11/20733-7, 13/11395-6)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
COÊLHO, Diego de Freitas. Development of anular centrifugal contactor and its application in the purification of bromelain from agroindustrial waste through unconventional aqueous two-phase systems. 2016. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Faculdade de Engenharia Química Campinas, SP.

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