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| Author(s): |
João Paulo Fernandes Vieira
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | Campinas, SP. |
| Institution: | Universidade Estadual de Campinas (UNICAMP). Faculdade de Engenharia Química |
| Defense date: | 2016-07-29 |
| Examining board members: |
Telma Teixeira Franco;
Carlos Eduardo Vaz Rossell;
Flavia Vischi Winck;
Edvaldo Rodrigo de Morais;
Roberto Ruller
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| Advisor: | Jose Geraldo da Cruz Pradella; Telma Teixeira Franco |
| Abstract | |
Rhodotorula glutinis CCT 2182, Rhodosporidium toruloides CCT 0783, Rhodotorula minuta CCT 1751 and Lipomyces starkeyi DSM 70296 were evaluated for the microbial lipids production. According to the lipid productivity and to the fatty acids profile, Rhodosporidium toruloides and Rhodotorula glutinis showed best results for bioconversion from Brazilian molasses. From these yeasts, the cultures were optimized according to the biomass and lipids productivity. The Rhodosporidium toruloides didn't reach high cell density, however the Rhodotorula glutinis showed high cell density cultivation. The average results for the maximum specific growth rate (?max), lipid yield (Pr) and the fraction of intracellular lipids were 0.25 h-1, 0.43 g l-1 h-1, and 55.3% respectively for Rhodotorula glutinis. Sulfuric acid and biomass fraction of 0.1:1 (w/w) with the temperature of 120ºC and 11.3 minutes of reaction was the best result for the cell lysis and lipids recovery. The mass balances, flow charts and economic analysis were performed with computational tool Superpro Design (Intelligent). The unitary costs for the yeasts biomass, microbial lipid and biodiesel were US$ 1,140.41, US$ 2,441.15 and US$ 2,665.20, respectively, considering fermenters built in carbon steel coated with epoxy. Within all the new technologies studied for biofuels production, the hemicellulose ethanol was the most promising economically. The microbial biodiesel production scenario is more attractive than ethanol from 1st generation, once the biodiesel prices were higher them US$ 2220.11/m3 (AU) | |
| FAPESP's process: | 12/00261-6 - Biotransformation routes of sugarcane carbohydates in long chain monoester with energy purposes |
| Grantee: | João Paulo Fernandes Vieira |
| Support Opportunities: | Scholarships in Brazil - Doctorate |