Effects of the solubility of yeast cell wall of Saccharomyces cerevisiae on digestibility, fermentation products on feces, and immunological parameters of adult dogs

Author(s)

Stephanie de Souza Theodoro

• Document type: Master's Dissertation
• Press: Jaboticabal. 2019-04-26.
• Institution: Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal
• Defense date: 2019-03-08
• Advisor: Aulus Cavalieri Carciofi; Thaila Cristina Putarov

Abstract

Derivates of yeast cell wall have been studied by its prebiotic effect. Recently, more purified and soluble preparations were developed, attempting to increase their biological actions. This study evaluated the inclusion of two yeast cell wall preparations, one conventional (YCW), and another with higher solubility of the mannan oligosaccharide fraction (YCWs), on their effects on nutrient digestibility, fermentation products on feces, and some immunological parameters of dogs. A single food formulation was used, unfolded on the following treatments: CON – control, without yeast cell wall addition; YCW – addition of 0.3% of a conventional yeas cell wall extract; YCWs – addition of 0.3% of a yeast cell wall extraction with elevated mannan oligosaccharides solubility. Twenty-four adult beagle dogs were used, eight dogs per food, distributed on a randomized block design. Blocks lasted 30 days, and tumor necrosis factor alpha and interleukins 6 and 10, ex vivo production of hydrogen peroxide and nitric oxide by peripheral neutrophils and monocytes, phagocytic index, and fecal IgA were evaluated at the beginning and ending of each period. Additionally, nutrient digestibility, feces production and quality, and fermentation products, pH, and biogenic amines were quantified on feces. Results were evaluated by variance analysis and compared by Tukey test (P<0.05). For the immunological parameters, the basal values were used as a covariate. The inclusion of YCWs reduced fat digestibility (P<0.05), increased the concentration of butyrate and putrescine, and reduced the lactate on feces (P<0.05), showing that mannan oligosaccharide solubilization resulted in higher fermentation of this compound, changing the metabolism of the microbiota of dogs. Lower IL-6 on serum was verified for dogs fed the YCWs diet (P<0.05), and the concentration of this cytokine also tended to be lower in dogs fed the YCW than the control diet (P=0.095), suggesting a reduction on the inflammatory activity of dogs. Higher phagocytic index was verified for peripheral monocytes after 30 days of the intake of the YCW food, indicating better innate immunity. In conclusion, both yeast cell wall derivates presented prebiotic effects on dogs, and the solubilization of the mannooligosaccharide fraction altered its interaction with the gut microbiota and biological effects on animals. (AU)

• FAPESP's process: 17/13623-7 - Mannanoligosaccharides from Saccharomyces cerevisiae cell wall on digestibility, fermentation products on feces, and immunological parameters of adult dogs
• Grantee: Stephanie de Souza Theodoro
• Support Opportunities: Scholarships in Brazil - Master