Surface characterization and antibiofilm efficacy in the use of 0.12% chlorhexidine digluconate on resins used in the preparation of contemporary provisional restorations, with and without application of light-curing glaze: In situ study

Author(s)

Betina Chiarelo Commar

• Document type: Master's Dissertation
• Press: Araçatuba. 2019-04-30.
• Institution: Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araçatuba
• Defense date: 2019-03-08
• Advisor: Marcelo Coelho Goiato; Daniela Micheline dos Santos

Abstract

During rehabilitation with ceramic fragments, it is important to use provisionalrestorations, which are essential to achieve clinical success and longevity of the definitive prosthesis, maintaining the health and protecting dental and periodontal tissues. The aimof this study was to evaluate colorstability, microhardness, roughness, and surface free energy of provisional prosthesissamples, with and without the application of light-curing glaze on the surface, and to evaluate the antibiofilm efficacy of 0.12% chlorhexidine digluconate in these samples.For this, resin samples of Bis-Acryl(Protemp, 3M ESPE, Sao Paulo, Brazil) and chemically-activated acrylic resin (CAAR)(Dencor, Artigos Odontológicos Clássico LTDA, Sao Paulo, Brazil)were made. Half of these samples were submitted to chemical polishing with light-curing glaze. These samples were placed in intraoral devices, whichcontained 4 niches(1 CAARwithout glaze, 1 CAARwith glaze, 1 Bis-Acrylwithout glaze, 1 Bis-Acrylwith glaze), used by volunteers (n=20). The experimental pases were: Thevolunteersinitially used the device containing the samples from the control group for seven days, in which 30% sucrose were applied. Then, theyused the device containing the samples from the test group for anotherseven days, in which 0.12% chlorhexidine digluconate and 30% sucrose wereapplied. Next, the tests of color change, microhardness, roughness and surface energy, initially and after theexperimentais phases, of all samples, and the biofilm was collected for later analysis of alkali-soluble extracellular polysaccharides (EPS).Data were submitted totheanalysis of variance (ANOVA)and to the Tukey test(p<0.05), except fortheEPSvalues, which were submitted to the ANOVA and Student-Newman Keuls (p<0.001). The test groups had lower color alterationin relation to their respective control groups, and there was a statistically significant difference only between the Bis-Acrylgroupswithout glaze. Regarding the microhardness, the values increased for the test groups in relation to their respective control groups, with a significant statistical difference in the final period, except for the CAARgroup without glaze, which had only a numerical increase,and the Bis-Acrylgroup without glaze, in which values decreased numerically. Comparing the test groupswith theirrespective control groups, the roughness values increased only numericallyfor the CAARgroup without glaze,in the final period. In the surface energy analysis, the values statistically increased for the test groups, when compared to their respective control group. The test groupspresented a statistically significant reductionof the biofilm amountin relation to the control groups. It can be concluded that the 0.12% chlorhexidine digluconate baserinsewas clinically satisfactory, significantly improving the properties of color change, microhardness, surface energy properties,and provided non-significantimprovementofthe roughness, thus contributing to a better clinical performance of the resins analyzed. The glaze application also contributed significantly to all the analyzed properties, except for the color change,in which it was deleterious. (AU)

• FAPESP's process: 17/11803-8 - Surface characterization and antibiofilm efficacy in the use of 0.12% chlorhexidine digluconate on resins used to manufacture contemporary temporary restorations, with and without the application of a light cured glaze
• Grantee: Betina Chiarelo Commar
• Support Opportunities: Scholarships in Brazil - Master