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| Author(s): |
Eriky Akio de Oliveira Tongu
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Botucatu. 2019-11-27. |
| Institution: | Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária e Zootecnia. Botucatu |
| Defense date: | 2019-09-09 |
| Advisor: | Marco Antônio Alvarenga |
| Abstract | |
Persistent mating-induce endometritis (PMIE) is a major cause of subfertility in the mare due to exacerbated uterine inflammation post breeding, so therapies have been studied to immunomodulate the inflammatory response. The objective of this work was to evaluate different concentrations of conditioned medium (MC) by equine mesenchymal stem cells on equine sperm kinetics and MC immunomodulatory capacity in uterine inflammation after breeding in resistant and susceptible mares as well as their fertility. Two experiments were performed. In experiment 1 the effect of the addition of different MC concentrations on the equine sperm kinetics and integrity was evaluated. In Experiment 2, the modulatory capacity of MC on the endometrial inflammatory process of resistant and susceptible mares after artificial insemination was evaluated, as well as its effect on conception rates. The MC did not significantly alter sperm kinetics and plasma membrane integrity of equine semen. Even in resistant mares MC reduced the percentage of neutrophils 6 hours after artificial insemination (p <0.05), not negatively interfering with fertility. In susceptible mares, MC decreased the percentages of neutrophils and intrauterine fluid 6 and 24 hours after AI (p <0.05). It was concluded that equine MC does not negatively affect the quality of equine semen or its fertility, modulating the inflammatory response after coverage in resistant and susceptible mares. (AU) | |
| FAPESP's process: | 18/03045-9 - Effect of the addition of conditioned medium by stem cells on the quality and fertility of equine semen |
| Grantee: | Eriky Akio de Oliveira Tongu |
| Support Opportunities: | Scholarships in Brazil - Master |