Microencapsulated sodium butyrate as an alternative antimicrobial additive in broiler diets challenged with Eimeria spp on intestinal integrity

Author(s)

Gabrieli Andressa de Lima

• Document type: Master's Dissertation
• Press: Dracena. 2020-02-14.
• Institution: Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Tecnológicas. Dracena
• Defense date: 2019-12-20
• Advisor: Valquíria Cação Cruz Polycarpo

Abstract

It is alleged that the use of antibiotics as growth promoters are related to microbial resistance, which has concern nutritionists and propels studies that evaluating microencapsulated sodium butyrate (MSB), for the purpose of improving the conditions of gastrointestinal tract. With the aim of evaluating the effect of MSB on performance, intestinal morphometry, count of oocysts excreted per gram of feces and intestinal mucosa carbon turnover of broilers challenged with Eimeria spp. 1.200 1-d-old male, Ross, allotted in a completely randomized design with 5 treatments and 6 replications. Treatments consisted of: T1 – Negative control, basal diet (BD); T2 - BD + 1,000 mg/kg (0.10%) of microencapsulated sodium butyrate (MSB); T3 -BD + 1,500 mg/kg (0.15 %) of MSB; T4 - BD + 2,000 mg/kg (0.20%) of MSB; T5 – Positive control, BD + antibiotic. No statistical differences were observed between the treatments for the variables of performance and intestinal morphometry in the studied periods. Therefore, it was noted that higher inclusions of MSB (1,500 and 2,000 mg/kg) showed to have potential when presenting higher values in average gain (AG), average feed intake (AFI), viability (VB), and production factor (PF), besides better feed conversion (F:G) and higher villus height, when compared to other levels of inclusion and the addition of antibiotics. In the oocyst count and lesion score analysis, it was observed statistical differences only for the studied collection periods, in which higher excretion was detected at 7 days after inoculation. The lesions caused per E. acervulina and E. tenella attenuated from the 10- and 8-day post-inoculation, respectively, and the major injuries caused per E. maxima were observed 5 days after inoculation. In the period of 0-21 days, the treatments that received additives (MSB or antibiotic) presented lower half-lives values, accelerating the turnover of the intestinal mucosa. In the period of 22-42 days, the treatments with the inclusion of 0.15% and 0.20% of MSB, and the treatment with antibiotics presented the highest half-lives values 35.29h, 32.92h and 38.27h, respectively. indicating that MSB was able to protect the intestinal mucosa from damage caused by Eimerias. It is concluded that the use of MSB in the diet doesn’t interfere in the performance of broilers, however, resembles the antibiotic in the maintenance of post-challenge intestinal mucosa with Eimeria spp. (AU)

• FAPESP's process: 18/10672-0 - Microencapsulated sodium butyrate as an alternative antimicrobial additive in broiler diets challenged with Eimeria spp on intestinal integrity
• Grantee: Gabrieli Andressa de Lima
• Support Opportunities: Scholarships in Brazil - Master