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| Author(s): |
Mayra Pereira Rocca
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | Botucatu. 2020-03-10. |
| Institution: | Universidade Estadual Paulista (Unesp). Faculdade de Medicina. Botucatu |
| Defense date: | 2020-02-19 |
| Advisor: | Helio Langoni; Renato Mancini Astray |
| Abstract | |
Vaccination against canine and feline rabies is the most effective prophylactic measure for preventing cases of human rabies. In Brazil, the domestic animal vaccination campaign is carried out annually, from the first 4 weeks of the animal's life. Available veterinary Rabies vaccines are composed of inactivated viruses with adjuvant. High level of neutralizing antibodies directly correlates with protection. Although Rabies vaccines have been available for many years, there is a clear lack of coverage due to the need for periodic boosters and potential problems with campaign-administered rabies vaccines, which makes many owners fail to meet their pet vaccination schedule. Thus, the development of new rabies vaccines should aim at increasing the safety of these vaccines and facilitating the booster administration process. The purpose of this study was to develop a vaccine prototype based on the use of a recombinant rabies virus glycoprotein (RVGP). Recombinant RVGP expressed in vivo by using a viral vector system (SFV-RVGP) or being expressed in a widely characterized S2 cell system, have shown immunogenic properties in preliminary experiments. Different preparations, combined or not with adjuvants, and different routes were tested in an established animal model. Immunogenicity evaluated by survival against intracerebral challenge and by serological tests (ELISA and seroneutralization). Results showed that both the RVGP produced in insect cells and the SFV-RVGP system were able to protect mice against viral challenge (CVS). The combination of adjuvants was important for the glycoprotein immunogenicity. Adjuvants as MPLA, silica (SBA-15) and ISCOM (immunostimulant complexes) were tested, with ISCOM showing more efficiency. (AU) | |
| FAPESP's process: | 16/07820-1 - Development of a new subunit veterinary vaccine against rabies |
| Grantee: | Mayra Pereira Rocca |
| Support Opportunities: | Scholarships in Brazil - Doctorate |