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| Author(s): |
Jéssica Luana Chechi
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | Botucatu. 2020-05-11. |
| Institution: | Universidade Estadual Paulista (Unesp). Instituto de Biociências. Botucatu |
| Defense date: | 2020-02-28 |
| Advisor: | Sandra de Moraes Gimenes Bosco |
| Abstract | |
Pythiosis, whose etiological agent is the oomycete Pythium insidiosum, is an lifethreatening disease that occurs mainly in tropical and subtropical countries, affecting several animal species, being more frequent in horses in Brazil and humans in Thailand. The disease is difficult to diagnose, because the pathogen's hyphae are often confused with Mucorales or Entomophthorales fungi in histological sections. In addition, there is no efficient and specific antigen that can be used for the rapid diagnosis and efficient treatment of pythiosis in different species. Antigens are molecules that the immune system recognizes, making these molecules important targets for the diagnosis of microorganisms. For the identification of antigens, immunoproteomics is considered a powerful tool. In this sense, we investigated which P. insidiosum antigens are recognized by horses in Brazil and Thai humans’ serum with pythiosis, in order to find similar proteins that could be used for future diagnostic tests or therapy approaches for pythiosis. To identify the immunoreactive proteins of P. insidiosum, immunoproteomic approaches were used, using the Western blot technique followed by the identification of antigens by mass spectrometry. Consequently, it was possible to identify 23 antigens recognized between the serum of horses and humans. Seven antigens were similar to the two types of serum, being heat shock cognate 70 KDa protein, heat shock 70 KDa protein, glucan 1,3-beta-glucosidase, fructosebisphosphate aldolase, serine/threonine-protein phosphatase, aconitate hydratase and 14-3-3 protein epsilon. Therefore, these results demonstrate that there are common antigens among the different serum tested and they may be excellent candidates for putative biomarkers for future diagnosis and new approaches for pythiosis therapy. (AU) | |
| FAPESP's process: | 16/10804-8 - Immunoproteomics of the oomycete Pythium insidiosum: antigens candidates for the diagnosis of equine pythiosis |
| Grantee: | Jéssica Luana Chechi |
| Support Opportunities: | Scholarships in Brazil - Doctorate |