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| Author(s): |
Marcella Rosa Filezio
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Jaboticabal. 2020-06-08. |
| Institution: | Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal |
| Defense date: | 2020-02-28 |
| Advisor: | Paola Castro Moraes |
| Abstract | |
Cataract is among the most common eye diseases in dogs. Phacoemulsification is the method that best fits its therapy. Among the complications related to this surgery, there are endothelial injuries that can induce permanent corneal decompensation, altering its transparency. The goal of this study was to evaluate the effects of ROCK inhibitor Y-27632 on endothelial protection in dogs undergoing phacoemulsification. Twenty eyes of 10 patients, male or female, aged 7 to 12 years, with bilateral senile cataract were used. Two groups were conceived: Control eyes (Oc) (n = 10), that received balanced saline solution (BSS) and Treated Eyes (Ot) (n = 10), which received intracamerally 0.3 mL of Y-27632 at a concentration of 100 µmol. / L, immediately after corneal suture. The corneal endothelium was assessed via non-contact specular microscopy at multiple time points before and after phacoemulsification. Cell density (cells/mm2 ) and area (µm2 ), corneal thickness (mm), hexagonality (%), and the coefficient of variation of cell size (%) were all assessed. P values equal to or less than 0.05 were considered significant. With respect to the density of endothelial cells, both groups showed losses, without statistical difference. There were no differences in corneal thickness and hexagonality. In Oc, the cellular area increased significantly between the evaluated moments, what was not observed in Ot. It is admitted that the intracameral application of 0.3mL containing 100µmol/L of Y-27632 during the phacoemulsification procedure in dogs under normal conditions, collaborates with the stability of the corneal endothelium cells, decreasing their loss (7.4%), compared to their non-use (11.8%). Keywords: canine, cataract, cornea, rho kinase (ROCK) (AU) | |
| FAPESP's process: | 18/02925-5 - Effects of intracameral Rho kinase inhibitor (rock, Y-27632) on the corneal endothelium of dogs undergoing phacoemulsification |
| Grantee: | Marcella Rosa Filezio |
| Support Opportunities: | Scholarships in Brazil - Master |