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| Author(s): |
Daniela Franchi Pereira da Silva Camilo
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | Campinas, SP. |
| Institution: | Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia |
| Defense date: | 2019-12-16 |
| Examining board members: |
Leonilda Maria Barbosa dos Santos;
Maria Elizabeth Rossi da SIlva;
Mônica Andrade Lima Gabbay;
Ronei Luciano Mamoni;
Elaine Conceição de Oliveira
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| Advisor: | Leonilda Maria Barbosa dos Santos; Walkyria Mara Gonçalves Volpini |
| Abstract | |
Objective: Characterization of partial remission using the insulin-adjusted HbA1c (IDAA1c) less or equal 9 definition in a multiethnic Brazilian population of children and adolescents with type 1 diabetes (T1D), in addition to the classification of the HLA class II model, autoantibodies and immune phenotype of regulatory T cells. Methods: We analyzed the prevalence of partial remission in 51 newly diagnosed T1D patients, with a mean follow-up of 13 months after diagnosis. For this study, HLA class II genotyping, anti-GAD65, anti-IA2 were considered. Peripheral blood cell flow cytometry was used to identify the regulatory T cell profile. Results: Partial remission occurred in 41.2% of DM1 patients within 3 months of diagnosis, especially in those aged 5 to 15 years. We have demonstrated a significant increase in the haplotypes of class II HLA DRB1*0301-DQB1*0201 in children and adolescents with a partial remission phase of the disease (42.9% vs 21.7% in non-remitters, p=0.0291). This haplotype was also associated with the reduction of anti-IA2 antibodies production. Homozygote DRB1*03-DQB1*0201 / DRB1*03-DQB1*0201 children had the lowest prevalence of IA-2A antibodies (p=0.0402). However, this association does not correlate with the time of remission phase. There was a tendency for the non-remitter T1D group to present lower frequency of CD4+CD25+ T cells compared to the partial remission group. The frequency of CD4+CTLA4+ T cells was lower in non-remitter T1D group compared to the partial remission group (0.95% vs. 1.65%, p <0.05). The DRB1*03-DQB1*0201 / DRB1*03-DQB1*0201 homozygotes tended to have a higher frequency of CTLA-4+ T cells than the other haplotypes. Conclusion: Although the number of patients studied was reduced, our data suggested that the association between genetics and decrease in antibody production to certain islet auto-antigen may contribute, at least in part, to the remission phase of T1D (AU) | |
| FAPESP's process: | 12/24667-1 - Functional study of cellular immunity in brazilians with type 1 diabetes at different stages of the disease and its integration with humoral markers |
| Grantee: | Daniela Franchi Pereira da Silva Camilo |
| Support Opportunities: | Scholarships in Brazil - Doctorate |