Screening for lipolytic enzyme codification genes in a metagenomic library of consortia specialized in diesel oil degradation.

Author(s)

Mariana Rangel Pereira

• Document type: Master's Dissertation
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2011-03-03

Examining board members

Eliana Gertrudes Macedo Lemos; Eleni Gomes; Vivian Helena Pellizari

• Advisor: Eliana Gertrudes Macedo Lemos

Abstract

Lipolytic enzymes have been attracting global market attention because they show enormous biotechnological potential. The present work was done as an attempt to find genes which codify lipolytic enzymes in a metagenomic library composed of diesel oil degradation microbe consortia. Clones were selected according to lipolytic activity and were then evaluated after cultivation in Petri dishes by observation of halo formation around the colonies. 30 clones produced halo formations and were identified as positives, two of which showed prominent results. These two were then selected and sub cloned. DNA from the sub libraries was sequenced, generating a complete contig for each clone. Using the ORF Finder five esterase/lipase ORFs were identified, with one of these attaining 58% of identity to a non cultivatable bacteria species. Assessment of the cladograms showed that two ORFs were similar to lipolytic enzyme family IV, while the other three ORFs were similar to family V. (AU)

• FAPESP's process: 09/06691-0 - Screening for lipolytic enzyme codification genes in a metagenomic library of consortia specialized in diesel oil degradation
• Grantee: Mariana Rangel Pereira
• Support Opportunities: Scholarships in Brazil - Master