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| Author(s): |
Camila Mira Sandy
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Ribeirão Preto. |
| Institution: | Universidade de São Paulo (USP). Faculdade de Medicina de Ribeirão Preto (PCARP/BC) |
| Defense date: | 2019-06-25 |
| Examining board members: |
Marcos de Carvalho Borges;
Pérsio Roxo Júnior;
Elaine Cristina Pereira de Martinis;
Jacques Robert Nicoli
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| Advisor: | Marcos de Carvalho Borges |
| Abstract | |
The prevalence of asthma has increased in children and communities that adopt a Western lifestyle and become more urbanized. Our previous results demonstrated that the administration of yeast, Saccharomyces cerevisiae UFMG A-905, attenuated asthma characteristics in an animal model. The theory of perinatal programming suggests that premature exposure to some stimuli exerts a protective effect throughout adult life. Objective: To evaluate the effects of perinatal administration of S. cerevisiae UFMG A- 905 in the prevention of asthma in the offspring of mice. Material and methods: S. cerevisiae UFMG A-905 was cultured in liquid YPD medium and administered to three groups of mice: before conception, during gestation and lactation (CGL group); during gestation and lactation (CG group); and only during lactation (LAC group). The offspring of these animals were then sensitized and challenged with ovalbumin (OVA). The control group received saline in the same periods. After, in vivo measurements of bronchial hyperresponsiveness (AHR) were performed. Total and differential cell count in bronchoalveolar lavage (BAL) and ELISA for IL-4, IL-5, IL-10, IL-13 and IL-17A in the lung homogenate or BAL and for OVA-specific IgE. Results: OVA group, compared to SAL group, presented a significant increase in AHR (p<0.0001), total number of cells (p<0.0001), eosinophils (p<0.001), macrophages (p<0.01), neutrophils (p<0.05) and levels of IL-5 (p<0.001) in BAL, IgE (p<0.05), and IL-13 (p<0.0001) in the lung homogenate. The animals of the CGL group, when compared to the OVA group, presented a significant reduction of AHR (p<0.01), levels of IL-5 (p<0.0001) in BAL and IL-13 (p<0.0001) and IL-4 (p<0.0001) in the lung homogenate. Serum IgE levels were significantly higher (p<0.05) in CGL group when compared to the OVA group. The GL group, when compared to the OVA group, presented a significant reduction in AHR (p<0.001), levels of IL-5 levels (p<0.001) in BAL and IL-4 (p<0.01) and IL-13 (p<0.01) in the lung homogenate. Serum IgE levels were significantly higher (p<0.01) in the GL group when compared to the OVA group. LAC group, when compared to the OVA group, presented a significant reduction in AHR (p <0.001), in the number of total cells (p <0.01), eosinophils (p <0.05), macrophages (p <0.05), levels of IL-5 (p <0.001) in BAL and IL-4 (p <0.05) and IL-13 (p <0.0001) in the lung homogenate. Conclusion: The administration of S. cerevisiae UFMG A-905 in the perinatal period decreased asthma-like characteristics and may be an alternative in the prevention of asthma. The lactation appears to be the more efficient period in modulating the offspring immune response (AU) | |
| FAPESP's process: | 17/10035-7 - Effects of perinatal administration of Saccharomyces cerevisiae UFMG A-905 on the prevention of asthma in the offspring of mice |
| Grantee: | Camila Mira Sandy |
| Support Opportunities: | Scholarships in Brazil - Master |