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| Author(s): |
Gabriel Gomide
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Campinas, SP. |
| Institution: | Universidade Estadual de Campinas (UNICAMP). Faculdade de Ciências Farmacêuticas |
| Defense date: | 2021-04-30 |
| Examining board members: |
Marcelo Lancellotti;
Daniel Fábio Kawano;
Selma Giorgio
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| Advisor: | Marcelo Lancellotti |
| Abstract | |
Gram-negative bacteria produce vesicles from the outer layer of their cellular membrane, which are known as outer membrane vesicles (OMVs). The biogenesis process of these OMVs are not completely elucidated but their role are related to DNA transfer mechanisms for transformation, inactivation of antibiotics and extracellular toxins, competitive advantage over a population, and as an alternative to model the structure of their membrane, regulating its composition due to external stresses. Also, it is known that there are proteins all over its surface that can work as antigens and molecular markers, just as in Neisseria meningitidis, whose OMVs are being used as vaccines against meningitis. Within that context, a search for optimization of OMV based vaccine had begun. A vaccine is composed by three parts: the antigen, which will stimulate a specific immune response against that pathogen; the carrier, an inert molecule that increases the rate by which antigen presenting cells absorb the vaccine; and adjuvants, auxiliary molecules responsible for inducing a strong and robust immunogenic response by itself. Because of this, nanomaterials have being studied due to its physical-chemical properties, mechanisms of interaction with biological systems and as potential candidates to novel vaccine formulations. Among them, carbon nanotubes and graphene are highlighted, and consequently, incorporated by healthcare researches and associated with vaccine to discover their effects. Due to its inertness, resistance, and factors that promote assembly and stability of biological molecules, its application as carriers was observed. However, other studies about its adjuvance were performed and their results indicated that its association with other molecules, or even the vaccine itself, stimulate and triggered an immune response, becoming potential adjuvants. The present project aims to to analyse the adjuvant properties that graphene and carbon nanotubes will have by associate with N. meningitidis OMV, and to compare its efficiency with adjuvants currently used (AU) | |
| FAPESP's process: | 18/07808-7 - Use of graphen and carbon nanotubes as adjuvants in vaccine based in outer membrane vesicles from Neisseria meningitidis |
| Grantee: | Gabriel Piccirillo Gomide |
| Support Opportunities: | Scholarships in Brazil - Master |