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| Author(s): |
Conrado de Campos Gonçalves
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Campinas, SP. |
| Institution: | Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia |
| Defense date: | 2014-02-14 |
| Examining board members: |
Marcelo Carnier Dornelas;
Gladys Flávia de Albuquerque Melo de Pinna;
Claudia Regina Baptista Haddad
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| Advisor: | Marcelo Carnier Dornelas |
| Abstract | |
The corona is a floral structure typical from the Passifloraceae family. The elucidation of the mechanisms responsible for the formation of the corona is of great importance to the comprehension of the evolutionary processes that allowed the diversification of the interactions with pollinators among the species of this family. This work aimed the identification and characterization of transcription factors (FT) preferentially expressed in the corona of Passiflora spp. For that, the expressed sequence tags (ESTs) database PASSIOMA was investigated and 139 cDNAs were identified: 69 derived from P. edulis flower libraries, 68 from P. suberosa and 2 from P. pohlii. Aiming to attribute identities to the sequences, a parsimony analysis was performed, with the obtaining of cladograms that allowed the identification of members belonging to 31 different transcription factor gene families in Passiflora spp. Macroarray experiments indicated that 9 putative genes coding for transcription factors showed expression in corona filaments. Further characterization of these transcription factors included RT-PCR experiments involving 5 out of the 9 genes and in situ hybridization with 2 out of the 5 genes that were previously analyzed with RT-PCR. The RT-PCR results indicated that these genes are expressed in corona tissues, but also in leaves and other floral organs. The in situ hybridization results confirmed that the genes studied are not specifically expressed in the corona (AU) | |
| FAPESP's process: | 11/14596-7 - Characterization of genes preferentialy expressed during corona development in Passiflora edulis Sims. (Passifloraceae) |
| Grantee: | Conrado de Campos Gonçalves |
| Support Opportunities: | Scholarships in Brazil - Master |