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| Author(s): |
Tedra Madeiral Mendonça
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Conjunto das Químicas (IQ e FCF) (CQ/DBDCQ) |
| Defense date: | 2002-10-04 |
| Examining board members: |
Paolo Di Mascio;
Josef Wilhelm Baader
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| Advisor: | Paolo Di Mascio |
| Abstract | |
Indole-3-acetic acid (IAA), a2 plant hormone, and its metabolite indole-3-carboxialdehyde (ICA) has been involved in several human pathologies as phenylketonuria and renal diseases. Formation of reactive oxygen species and electronically excited states as singlet oxygen (1O2) and triplet carbonyl (R2-C=O*), during the aerobic oxidation catalyzed by horseradish peroxidase (HRP) has been reported to be involved in the IAA cytotoxic effects. Our results show an increase in the formation of 8-oxo-7,8-dihydro-2\'deoxiguanosine (8-oxoxdGuo) after IAA/HRP/O2 system treatment in vitro as inferred from high performance liquid chromatography-electrochemical detection (HPLC-EC) measurements. Treatment of mammalian cells (CV1-P and neutrophils) with the system induces 8-oxoxdGuo formation in DNA as well increased levels of lipid peroxidation CV1-P cells when compared to controls. The 1,N2-etenodeoxyguanosine was detected in neutrophils DNA. Therefore, in this study, we presented evidence of biomolecule damage by IAA/HRP/O2 system. (AU) | |