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| Author(s): |
Luciana Tendolini Brito
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2015-10-29 |
| Examining board members: |
Elizabeth Natal de Gaspari;
Waldely de Oliveira Dias;
Eliana Faquim de Lima Mauro
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| Advisor: | Elizabeth Natal de Gaspari |
| Abstract | |
In the first part of the study Swiss mice were immunized by different routes of immunization with OMVs of Neisseria meningitidis with DDA-BF or HA as adjuvants. Adjuvants and different routes were compared regarding immune responses through ELISA, Immunoblot, HTT and histopathological analysis. Animals immunized with only adjuvants did not produce evidence of antibodies. After single dose and 15 days, of immunization with HA presented antigen specific IgG higher in relation to the DDa-BF in the subcutaneous, intraperitoneal and intramuscular immunization . After 2 doses and 66 days, all exhibited IgG, and the bonds that received the HA with OMVs produced titles discreetly higher and still high levels of antibodies. The profile of the immune response to Th1/Th2 pattern has been evaluated. Both adjuvants promoted the production of IgG2a, the responses varied according to the immunization routes used. While the subcutaneous and intramuscular routes induced similar titles of IgG2a to both adjuvant intraperitoneal route with had highest title. IgG1 production was modulated only by HA, being more robust in subcutaneous injection, followed by intramuscular with values very close to those of intraperitoneal. Isogenic Balb/c and C57Bl/6J H2d H2b mice were immunized by subcutaneous administration. Been evaluated antibody production of type IgG, IgG1 and IgG2a, as well as the IgG avidity index. In General, the greater production of OMVs HA induced antibodies that OMVs DDA or just OMVs, while the controls .DDA-BF and controls showed no antibody levels. The techniques used in the study did not observe a significant difference between the two adjuvants used independent of the route and of mice strains used. (AU) | |
| FAPESP's process: | 13/11147-2 - Study of the immunogenicity of outer membrane antigens of Neisseria meningitidis and interaction with cationic lipids: evaluation of different immunization schedules in different strains of mice |
| Grantee: | Luciana Tendolini Brito |
| Support Opportunities: | Scholarships in Brazil - Master |