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| Author(s): |
Alana Caroline Costa
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Faculdade de Medicina (FM/SBD) |
| Defense date: | 2017-06-19 |
| Examining board members: |
Wagner Farid Gattaz;
Valdemir Melechco Carvalho;
Beny Lafer;
Michael Murgu
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| Advisor: | Wagner Farid Gattaz |
| Abstract | |
Phospholipids and related molecules comprise 60% of the non-aqueous portion of the brain and are the major constituents of neuronal and glial cell membranes. Phospholipids are essential for all living cells and therefore changes in their metabolism can influence the organism. Changes in phospholipid metabolism are known to be involved in numerous neuropsychiatric disorders including Alzheimer\'s disease, schizophrenia and bipolar disorder. In this way, we aimed to understand the lipid composition of membrane-related metabolites of patients with different neuropsychiatric diseases. For this, we use Flow Injection Analysis (FIA) coupled with mass spectrometry, a robust analytical methodology that provides a complete profile of the substances in complex matrices. To interpret the results, we chose to perform the CART method - Classification and Regression Tree. We found 4 metabolites that are able to distinguish TB patients from patients with SCZ and 3 other metabolites that together are able to differentiate individuals with CCL and AD. These results show us the potential of membrane phospholipids as diagnostic biomarkers, which may aid in the diagnostic confirmation and elucidation of pathophysiological mechanisms of the diseases studied (AU) | |
| FAPESP's process: | 14/20913-3 - Determination of plasma phospholipids in neuropsychiatric disorders |
| Grantee: | Alana Caroline Costa |
| Support Opportunities: | Scholarships in Brazil - Master |