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| Author(s): |
Bruno Vecchiatto
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Escola de Artes, Ciências e Humanidades (EACH) |
| Defense date: | 2019-08-06 |
| Examining board members: |
Fabiana de Sant'Anna Evangelista;
Felipe Santiago Chambergo Alcalde;
Lígia de Moraes Antunes Corrêa;
Edilamar Menezes de Oliveira
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| Advisor: | Fabiana de Sant'Anna Evangelista |
| Abstract | |
The renin angiotensin axis is composed by the angiotensin converting enzyme 2 (ACE2), angiotensin 1-7 (Ang1-7) and receptor Mas (ACE2/Ang1-7/Mas) is associated with lowering adiposity, higher insulin sensitivity and glucose uptake. In this study we investigated the association of ACE2/Ang1-7/Mas axis on skeletal muscle for the prevention of metabolic disturbance by the aerobic endurance training (AET). Mice C57BL6/J were separated in sedentary group and chow diet (C, n=10); trained and chow diet (T, n=9); sedentary and cafeteria diet (CAF, n=9) and the trained group with cafeteria diet (CAFT, n=10). The AET consisted of 60 minutes of running at 60% of the maximal velocity, 5x/wk for eight weeks. The trained groups had lower body mass after AET. On maximal effort test, T group increased significantly the VO2max, and both trained groups presented higher velocity and time, higher velocity when VO2max was achieved and lower relative running cost. On soleus muscle, trained groups increased the enzymatic activity of citrate synthase and β-HAD. CAFT group presented the mitochondria on the subsarcolemmal area with more severe damages when compared to T group, and the CAF group presented lower moderate damage in intermyofibrillar area when compared to C group. There were no differences on Tfam, NRF-1 and COXII gene expression, on mitochondrial dynamics protein expression and on the ACE2/Ang1-7/Mas axis on soleus and gastrocnemius muscles. In conclusion, the AET prevented the increase in body mass induced by cafeteria diet, improved the aerobic capacity and promoted the increase in oxidative enzymes activity on soleus muscle. However, the adaptations induced by AET were not associated with ACE2/Ang1-7/Mas modulation on skeletal muscle (AU) | |
| FAPESP's process: | 16/20659-5 - The role of ACE2/Ang-(1-7)/Mas axis on skeletal muscle for prevention of metabolic disorders by aerobic training |
| Grantee: | Bruno Vecchiatto |
| Support Opportunities: | Scholarships in Brazil - Master |