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| Author(s): |
Janaina da Silva Vieira
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Escola de Educação Física e Esportes (EEFE/BT) |
| Defense date: | 2020-02-19 |
| Examining board members: |
Patricia Chakur Brum;
Dawit Albieiro Pinheiro Gonçalves;
Carlos Roberto Bueno Júnior;
Carlos Ugrinowitsch
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| Advisor: | Patricia Chakur Brum |
| Abstract | |
Pulmonary hypertension (PH) is a chronic syndrome and the major cause of right heart failure non-related with left ventricular dysfunction, with a prevalence of 50% in patients with right heart failure (HF) (HF with preserved left ventricular ejection fraction). Right HF can culminate in muscle atrophy that is directly related to the poor prognosis of HF patients as it contributes to exercise intolerance. However, there is a gap in the literature regarding the right HF phase that skeletal myopathy is stablished and effective strategies to minimize the loss of muscle mass and function on PH-induced right HF. In recent decades, the number of evidences supporting aerobic exercise training (AET) as an important secondary treatment for HF has increased. In fact, previous work of our group has shown that AET minimizes skeletal myopathy in both patients and experimental models of HF with left ventricular dysfunction. However, the potential effect of AET on the pathophysiology of HF is not yet fully understood, as this intervention has been prescribed only a few years ago for right HF. There are several HF models used to better understand left HF in mice, but there is still a lack of good models to study right HF in mice. Thus, the aim of this project was to characterize a robust model of monocrotaline (MCT)-induced right HF, as well as to evaluate the impact of AET on skeletal and cardiac muscle of mice with right HF. We have used C57BL6 / J mice treated with (MCT) or saline. The treatment with MCT for two months induced right ventricular dysfunction paralleled by pulmonary edema, hypertrophy without affecting left ventricle or exercise tolerance compared with saline treated group, which characterizes an isolated right HF at this stage of the syndrome. Four months of MCT treatment caused right and left ventricular dysfunction with pulmonary edema associated with exercise intolerance and atrophy of type II fibers compared with saline group. Therefore, at this stage, the right HF is severe and compromises left ventricle function leading to exercise intolerance and skeletal myopathy. To better understand the effects AET on PH-induced right HF, animals treated with MCT for 4 months were submitted to AET in the last 4 weeks of treatment. AET was effective in improving left ventricular function and exercise tolerance on MCT group, as well as restoring cardiac and skeletal muscle trophicity, being an interesting strategy for the treatment of skeletal myopathy in PH-induced right HF (AU) | |
| FAPESP's process: | 16/24284-6 - Role of aerobic physical training in muscle plasticity in experimental model of heart failure induced by pulmonary hypertension |
| Grantee: | Janaina da Silva Vieira |
| Support Opportunities: | Scholarships in Brazil - Master |