Physiology of Saccharomyces cerevisiae strains isolated from brazilian biomes: new insights into biodiversity and industrial applications.

Author(s)

Felipe Baratho Beato

• Document type: Master's Dissertation
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2016-06-09

Examining board members

Andreas Karoly Gombert; Sandra Regina Ceccato Antonini; Aldo Tonso

• Advisor: Andreas Karoly Gombert

Abstract

During industrial fuel ethanol production, the commercial Saccharomyces cerevisiae strains introduced in the process are naturally substituted by wild strains, whose origin is unknown. With the aims of gaining insight into this issue, of getting a better understanding on the natural habitat of S. cerevisiae, and also of identifying industrially relevant phenotypes, we performed a physiological and genetic characterization of 14 indigenous S. cerevisiae strains, isolated from Atlantic Forest and Cerrado biomes in Brazil. Cultivations were performed under standard laboratory conditions, with or without stress (high temperature, acid or ethanol), and also under conditions mimicking the industrial environment. As a main result, we identified S. cerevisiae UFMG-CM-Y257 as a strain displaying high specific growth rate (0.57 ± 0.02 h-1), high ethanol yield (1.65 ± 0.02 mol ethanol mol hexose-equivalent-1), high ethanol productivity (0.19 ± 0.00 mol L-1 h-1), high acetic acid tolerance (10 g L-1) and high temperature tolerance (40 oC). (AU)

• FAPESP's process: 13/17859-4 - Growth physiology in bioreactor cultivations of Saccharomyces cerevisiae strains isolated from Brazilian biomes
• Grantee: Felipe Baratho Beato
• Support Opportunities: Scholarships in Brazil - Master