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| Author(s): |
Fernando Suzigan Nobre
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2013-03-26 |
| Examining board members: |
Lara Durães Sette;
Welington Luiz de Araujo;
Rafaella Costa Bonugli Santos
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| Advisor: | Lara Durães Sette |
| Abstract | |
Cold-active lipases are widely distributed in micro-organisms which survive in low temperatures, in this context; fungal isolation from Antarctic environment can be considered strategic for obtaining lipases at low temperatures. The present work aimed to assess the production of lipases and diversity of filamentous fungi isolated from samples of the Antarctic environment. A total of 253 isolates were retrieved from 10 marine and four terrestrial samples collected on the Antarctic continent, amongst then 68 presented positive results and were subjected to the experiments of lipase production evaluation and biodiversity characterization. Lipolytic fungi were identified as representatives of the genera: Geomyces, Penicillium, Cosmospora, Thelebolus, Chaetomium, Hypocrea, Antarctomyces, Mortierella and Cadophora. In addition, some isolates of the same ribotype were affiliated to the order Mucorales, and another one, showed to be a putative new species from phylum Basidiomycota. The fungus Geomyces pannorum AL1-1B was selected as the lipase best producer and showed activity of 761.68 U/L in the 5th day of cultivation at pH 8.0 at 15 °C, highlighting the biotechnological potential for lipase production at low temperatures. (AU) | |
| FAPESP's process: | 09/12010-5 - Lipolytic activity and biodiversity of Antartic-derived filamentous fungi |
| Grantee: | Fernando Suzigan Nobre |
| Support Opportunities: | Scholarships in Brazil - Master |