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| Author(s): |
Sérgio Paulo Dejato da Rocha
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2010-08-13 |
| Examining board members: |
Waldir Pereira Elias Junior;
Tania Aparecida Tardelli Gomes do Amaral;
Solange Barros Carbonare;
Luis Carlos de Souza Ferreira;
Maria Leonor Sarno de Oliveira
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| Advisor: | Waldir Pereira Elias Junior |
| Abstract | |
aEPEC is capable to cause A/E lesion, triggered by proteins encoded by LEE region. We analyzed structurally and functionally the LEE region of aEPEC strains displaying LAL, AA, DA, and one nonadherent (NA) strain. The adherence characteristics and ability to cause A/E were investigated in epithelial cells. The displayed adherence patterns were independent of the cell line origin. A/E lesion was detected in some cellular lines after contact only with ALL- and AD-strains. LEE region presence was detected intact and real time PCR, microarray and immunodetection, in all samples tested. An EspFu-expressing plasmid was introduced in all strains, demonstrating no influence of this protein neither in the adherence patterns nor in the capacity to cause A/E of the LAL-, AA- and DA-strains. But, NA-strain expressed the LAL pattern and was able to cause A/E. Therefore, EspFu was shown to play a role in cell adhesion in addition to the establishment of the A/E lesion in vitro. (AU) | |