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| Author(s): |
Nidia Alice Pinheiro
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Conjunto das Químicas (IQ e FCF) (CQ/DBDCQ) |
| Defense date: | 2001-10-01 |
| Examining board members: |
Ricardo Renzo Brentani;
Hamza Fahmi Ali El Dorry;
Bryan Eric Strauss;
Sergio Verjovski Almeida;
Marco Antonio Zago;
Bianca Silvana Zingales
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| Advisor: | Ricardo Renzo Brentani |
| Abstract | |
Colon tumorigenesis is considered a complex multistep process that occurs through a series of gene mutations, leading from precursor lesions to invasive and metastatic adenocarcinoma. Toe development and progression of cancer and the reversal of tumorigenicity are accompanied by complex changes in patterns of gene expression. Microarrays of cDNA provide a powerful tool for studying these complex phenomena. The purpose of the present study is to determine differential gene expression in colon tumors as compared with normal tissue. We also investigated the possible variability of the gene expression among pools of micro dissected colon tumors. These samples were analyzed using the Gene Discovery Array Human system (Incyte Genomics) that consists of two nylon membranes in a double spotted pattern at a density of 36,864 per spots per filter or 18,376 individual cDNA clones. Our results showed several differentially expressed clones between normal and tumor tissue analyzed. Our results were compared by software analysis to the patterns was found by Alon et al., 1999 using affymetrix oligonucleotide array complementary to more than 6,500 human genes tumor. We analyzed ours data against SAGE library by \"Northern Virtual\" tools (\"Sagenet.org\" homepage). Some genes founded in these analyses were tested in RT-PCR semi-quantitative analysis. (AU) | |