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| Author(s): |
Rodrigo Pinheiro Araldi
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2017-12-04 |
| Examining board members: |
Rita de Cassia Stocco;
Rodrigo Franco de Carvalho;
Janete Maria Cerutti;
Elizabeth Natal de Gaspari;
Paulo Luiz de Sá Junior;
Edislane Barreiros de Souza
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| Advisor: | Rita de Cassia Stocco |
| Abstract | |
BPV metastatic action remains unclear. This study evaluated the BPV action on epithelial-mesenchymal transmition (EMT), using cell lines form papilloma (P), fibropapilloma (FB) and esophageal carcinoma (EC). Results showed the productive infection presence and the proliferative potential increase in these cells. However, it was verified the mitochondrial membrane potential loss in relation to normal skin (control) and the oxidative stress increase as result of E6 oncoprotein, justifying the clastogenicity and stem-cell phenotype acquisition described in P, FB and EC cells. This cell lines showed a migratory capability as result of cytoplasmic sequester of E-cadherin, and the increase levels of vimentin, vinculin and N-cadherin as consequence of STAT3 and SLUG factors activation, suggest the virus action on EMT. These results were also verified in tissue samples, reinforcing the BPV action on EMT, as well as demonstrating the potential of these cell lines as model to study the metastasis. (AU) | |
| FAPESP's process: | 14/20617-5 - Study of the action of bovine papillomavirus (BPV) in epithelial-mesenchymal transition |
| Grantee: | Rodrigo Pinheiro Araldi |
| Support Opportunities: | Scholarships in Brazil - Doctorate |