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| Author(s): |
Renata Barbosa
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | Campinas, SP. |
| Institution: | Universidade Estadual de Campinas (UNICAMP). Faculdade de Ciências Médicas |
| Defense date: | 2020-02-20 |
| Examining board members: |
Fernando Cendes;
Fábio Rogério;
Enrico Ghizoni;
Marino Muxfeldt Bianchin;
Carla Alessandra Scorza
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| Advisor: | Fernando Cendes |
| Abstract | |
The hippocampus is the brain region most investigated in patients and experimental models of epilepsy. Hippocampal sclerosis is particularly associated with temporal lobe epilepsy and comprises a set of changes that occur during the epileptogenic process as a result of an initial insult and still discussed in the central nervous system. Indeed, the pathophysiological mechanisms involved in this process remain unclear. The present study aimed to evaluate strucutural and metabolic changes in the dorsal hippocampus of Wistar rats using neuroimaging techniques before and after induction status epilepticus with pilocarpine. The animals were divided into two groups: sham group (n=13) and pilocarpine group (n=25). Baseline and subsequent images were acquired at 48 hours, 15- and 30-days post-treatment using a 3 Tesla scanner (Philips) and a special coil for rats. T2-weighted, diffusion tensor (DTI), and metabolic and biochemical (proton magnetic resonance spectroscopy) images were acquired. The mortality rate after pilocarpine-induced status epilepticus was 41.67%. Neuroimaging data demonstrated damage to the dorsal hippocampus after pilocarpine-treatment. These damages were characterized by hippocampal volume reduction, decreased in fractional anisotropy 15 days post-treatment and increased in radial diffusivity 15- and 30-days post- treatment suggesting neuronal damage, demyelination and axonal damage. Metabolic levels of N-acetyl-aspartate + N-acetyl-aspartyl-glutamate/Creatine and Glutamate + Glutamine/Creatine were reduced at 48 hours post-treatment, which may indicate neuronal damage and/or dysfunction of energy metabolism. Therefore, our findings demonstrated significant damage in the dorsal hippocampus; showing longitudinal structural and metabolic changes after pilocarpine-treatment (AU) | |
| FAPESP's process: | 14/11277-6 - Structural and biochemical evaluation by magnetic resonance imaging in temporal lobe epilepsy model in rats with and without status epilepticus |
| Grantee: | Renata Barbosa |
| Support Opportunities: | Scholarships in Brazil - Doctorate |