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| Author(s): |
Fernanda Kaiser Lima Krenchinski
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Botucatu. 2021-09-22. |
| Institution: | Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária e Zootecnia. Botucatu |
| Defense date: | 2021-07-16 |
| Advisor: | José Roberto Sartori |
| Abstract | |
Heat stress causes many damages in industrial poultry farming and in order to minimize them, nutritional strategies have been adopted, with emphasis, mainly on natural foods. The objective of this work was to study the effect of passion fruit seed oil (OSM) supplementation on immunity and immune responses, meat quality and antioxidant system of broiler chickens. For this, 450 1-day-old male chicks of the Cobb® 500 strain were used, housed in galvanized wire cages, in two chambers with 45 cages each. The design was completely randomized, in a 2 x 5 factorial scheme, with two temperatures (thermoneutral and cyclic heat stress) and five treatments (control without inclusion of OSM + four levels of inclusion of OSM (0.30; 0.50; 0) .70; 0.90%) with nine replicates of five birds each.The ration was devoid of antibiotics as a performance enhancer, but added anticoccidial in all treatments. Data were analyzed using analysis of variance with the aid of SAS 9.3 (SAS Institute, 2008) and, being significant (P<0.05) in the mean test by the Tukey test. which had higher values in relation to heat stress, and abdominal fat had lower value for heat challenged birds. OSM influenced the higher weight (in grams) of the legs. Broilers reared under heat stress had lower value a* in the breast musculature than broilers raised in a thermoneutral environment. Regarding proteomic twodimensional electrophoresis analysis in polyacrylamide gel (2D-PAGE) of blood plasma for meat quality results, the protein Actin alpha skeletal muscle showed a significant difference in treatments with inclusion of 0.9% OSM in both temperatures (heat stress and thermoneutral) and, Neuropilin-1, in thermoneutral treatment with 0% inclusion of OSM. At 21 days of age, a significant interaction for antibody titration was observed, and broilers reared in a stress environment that received the level of 0.90% of OSM had a higher value of vaccine titration. At 28 days of age, again the animals that received 0.90% inclusion of OSM and, regardless of the rearing environment, had higher antibody titers. In the proteomic analysis of blood plasma (2D-PAGE) several proteins that are related to the immune system of broiler chickens were found: Complement factor B-like protease (Fragment); Glyceraldehyde-3-phosphate dehydrogenase; Mannose-binding protein; Zinc finger protein ubi-d4; Ig lambda chain C region; Ig lambda chain V-1 region; Apolipoprotein A-I. Proteomic analysis also showed results regarding the maintenance of the gastrointestinal epithelium, with the differential expression of Retinol-binding protein 4 and Homeodomain-only protein. There was a greater % reduction of DPPH with the greater inclusion of OSM, as well as greater expression of Alcohol dehydrogenase 1, in treatments with greater inclusion of OSM. There was also a significant difference for the temperature effect on the catalase enzyme activity, which was higher in a thermoneutral environment. As well as, Nuclear receptor coactivator 7 which was differentially expressed in the 0% treatment of broilers reared in thermoneutral environment. Hemoglobin subunit epsilon had its expression differentiated in the treatment of 0.9% of broilers reared in stress environment. Furthermore, several protein spots related to molecular chaperones were differentially expressed in the 0.5% and 0.9% OSM inclusion treatments at both rearing temperatures. Membrane-associated progesterone receptor component 1 was differentially expressed in treatments of 0.9% inclusion of OSM in both rearing environments. (AU) | |
| FAPESP's process: | 19/12179-1 - Study of plasma proteomic and antioxidant response of broilers supplemented with passion fruit seed oil |
| Grantee: | Fernanda Kaiser de Lima Krenchinski |
| Support Opportunities: | Scholarships in Brazil - Master |