Differentiation of Mycoplasma spp. isolated from milk samples from clinical mastitis cases and bulk tanks milk.

Author(s)

Bruna Churocof Lopes

• Document type: Master's Dissertation
• Press: Botucatu. 2020-08-31.
• Institution: Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária e Zootecnia. Botucatu
• Defense date: 2020-08-25
• Advisor: Helio Langoni

Abstract

Mastitis is the main affection that affects cattle destined for milk production, it generates losses to the producer and the industry due to the loss in production and lower yield in the industrial processing of derivatives. It consists of a disease with a multifactorial character of multiple etiology including microorganisms such as bacteria, viruses, fungi and algae. Among the most prominent bacterial microorganisms are Staphylococcus aureus and non-aureus, all characterized as contagious, in addition to Corynebacterium bovis and Streptococcus agalactiae. Mycoplasma spp. Is highly contagious, with emphasis on M. bovis, M bovigenitalium and M. californicum pathogens considered important in other countries, however, less studied in Brazil. Due to the high degree of contagiousness of the agent and the economic aspects, it is important to monitor the occurrence of mastitis caused by Mycoplasma spp, as well as the differentiation of the species involved for the adoption of disease prevention and control measures on the property. The objective of the present study was to investigate the presence of mycoplasmas, bacteria of the genus Mollicutes, and to differentiate species in milk samples from cows with clinical mastitis, and as a screening for milk samples from expansion tanks (subclinical mastitis), the from molecular techniques, microbiological culture of PCR positive samples and genetic sequencing. Of the 993 samples analyzed, 136 positive samples were found for the Mollicutes class using the concentration technique by means of centrifugation prior to DNA extraction and, using the same samples, only 80 were positive performing the extraction directly from milk, showing the importance of implementation of the technique in the routine of molecular analyzes of milk samples for investigation of bacteria of the Mollicutes class. No positive samples were found for the species tested, M. bovis, M, bovirhinis, M. bovigenitalium, M. californicum and M. alkalescens (AU)

• FAPESP's process: 18/09086-9 - Differentiation of species of Mycoplasma spp. isolated from cases of clinical mastitis cases and expansion tanks
• Grantee: Bruna Churocof Lopes
• Support Opportunities: Scholarships in Brazil - Master