Mastitis is the main condition affecting livestock destined to milk production, which causes losses to the producer and industry, the loss of production and lower yields in the industrial processing of derivatives. It is a multifactorial disease of multiple etiology including microorganisms such as bacteria, viruses, fungi, yeasts and algae. Among the most prominent bacterial microorganisms are coagulase-positive (SCP) and negative (SCN) staphylococci, the main Staphylococcus aureus (SCP) and several SCN species, all characterized as contagious, in addition to Corynebacterium bovis and Streptococcus spp. Mycoplasma spp., with emphasis on M. bovis, M. californicum and M. bovigenitalium pathogens considered important in other countries (Kobayashi, 1998), are less contagious. The objective of the present study is to evaluate the presence of bacteria of the Mollicutes class, and to carry out the species differentiation in milk samples from cows with clinical mastitis and, as a screening, for milk samples from expansion tanks (subclinical mastitis) from of molecular techniques, microbiological culture of the positive samples in the PCR and genetic sequencing.Due to the high degree of contagiousness of the agent and its economic losses, it is important to monitor the occurrence of mastitis caused by Mycoplasma spp, as well as the differentiation of the species involved and the adoption of disease prevention and control measures in the property.
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