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Genetic heterogeneity of Anopheles darlingi and its implications for malaria epidemiology

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Melina Aulino Campos Lima
Total Authors: 1
Document type: Doctoral Thesis
Press: Botucatu. 2016-07-28.
Institution: Universidade Estadual Paulista (Unesp). Instituto de Biociências. Botucatu
Defense date:
Advisor: Paulo Eduardo Martins Ribolla

Forest degradation, human environmental alteration and climate changes are all influence anopheline populations. Anopheles darlingi is the main vector of malaria parasite in Brazil and other countries of South America. Deforestation and others anthropogenic activities have been accompanied by sharp increases in both abundance of the primary malaria vector Anopheles darlingi and numbers of malaria cases. Besides it is widely distributed, this species display great behavioral plasticity and morphological, biological and genetic diversity. The aim of this study was to analyze population genetic diversity of An. darlingi related to geographical distribution, seasonal dynamics and hematophagic behavior, using microsatellites and SNPs markers. An. darlingi specimens were collected in two close rural settlements and in an urban area about 600 km away. In addition, collections were performed in both semesters of the year and, indoor and outdoor during the biting activity period of the vector, i.e., 6pm-6am. The results showed subpopulations of An. darlingi related to geographical aspect, in a macro and micro geographic scales, better accessed with SNPs dataset. Moreover, this study corroborated with a previous finding showing two subpopulations of An. darlingi related to rainfall. Finally, for the first time, genetic heterogeneity was found in sympatric populations of this vector, and it was associated with a phenotype of hematophagic behavior, endo and exophagy. These outcomes demonstrate that genetic heterogeneity may represent an important vector phenotypic variation with potentially highly significant consequences for malaria transmission. Since each site has unique environmental characteristics and therefore, different population compositions of vectors, specific interventions on a smaller scale become interesting approaches for optimal targeted malaria transmission interventions. (AU)

FAPESP's process: 12/04881-9 - Population dynamics of Anopheles darlingi by genotyping microssatellite in Acrelândia- AC - Brazil
Grantee:Melina Aulino Campos de Lima
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)