Evaluation of redox status, inflammatory response, telomeres and gene expression in anesthesiologists

Author(s)

Kátina Meneghetti Souza

• Document type: Doctoral Thesis
• Press: Botucatu. 2020-05-27.
• Institution: Universidade Estadual Paulista (Unesp). Faculdade de Medicina. Botucatu
• Defense date: 2020-02-19
• Advisor: Mariana Gobbo Braz

Abstract

The current study aimed to evaluate oxidative stress, inflammatory response, the expression of related genes and telomere length in anesthesiologists. This crosssectional study was conducted at the Botucatu Medical School Hospital, São Paulo State University - UNESP, in 60 physicians, allocated in two groups, as follows: 30 anesthetists occupationally exposed to waste anesthetic gases (WAG) - isoflurane, sevoflurane, desflurane (average of 10 parts per million - ppm), and nitrous oxide (150 ppm), and 30 physicians exposed-free, who were matched by age, sex and lifestyle. Oxidative stress was analyzed by systemic oxidative DNA damage, lipid peroxidation, nitric oxide metabolites, antioxidant defense and individual lipophilic antioxidants, and complex B vitamins and homocysteine. The inflammatory response was evaluated by pro-inflammatory interleukins (IL6, IL8 and IL17A), Creactive protein, and liver enzymes. Telomere length and the expression of genes related to repair of oxidative DNA damage (hOGG1 and XRCC1), antioxidant defense (NRF2), genome maintenance (TP53) and inflammation (IL6, IL8 and IL17A) were evaluated by real-time quantitative polymerase chain reaction. There was no significant difference between groups for the evaluated parameters, except for IL8 gene expression, which was higher in the exposed group (p = 0.04). Occupational exposure to commonly used halogenated, along with nitrous oxide, is not associated with oxidative stress, micronutrients, systemic inflammatory response and genetic instability, but is associated with molecular modulation of inflammation (IL8 proinflammatory gene overexpression) in anesthesiologists working in a university hospital, suggesting that this transcript is a biomarker of ocuppational exposure to WAG. It is prudent and necessary to reduce WAG occupational exposure in the workplace and to monitor all exposed professionals. (AU)

• FAPESP's process: 16/23902-8 - Evaluation of redox status, inflammatory response, telomeres and gene expression in anesthesiologists
• Grantee: Kátina Meneghetti de Souza
• Support Opportunities: Scholarships in Brazil - Doctorate