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| Author(s): |
Pâmela Soto Garcia
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Medicina Tropical de São Paulo (IMT) |
| Defense date: | 2018-12-13 |
| Examining board members: |
Fabio de Lima Leite;
Renan Barros Domingues;
Hiro Goto;
Marcelo de Assumpcao Pereira da Silva
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| Advisor: | Fabio de Lima Leite; Doralina Guimarães Brum Souza |
| Abstract | |
Myelin oligodendrocyte glycoprotein (MOG) and myelin basic protein (MBP) have been implied as the most important target antigens in demyelinating processes of central nervous system (CNS) and the most important antigens candidates whom arised from the animal model for multiple sclerosis (MS), the experimental autoimmune encephalomyelitis (EAE). The first autoantibodies detected in serum and cerebrospinal fluid (CSF) of MS patients were antibodies against myelin antigens. Differential diagnostic to MS includes the presence of oligoclonal bands (OCBs) in CSF and absence in serum, which demonstrate intrathecal IgG synthesis. Most applied techniques to detection of antibodies nowadays are ELISA, cell-based assay and western blot (WB). In this context, the study of anti-MOG role in e disease may be supported through its detection by atomic force spectroscopy (AFS) technique. AFS is a highly sensitive technique that allows molecular detection as a functionalized atomic force microscope (AFM) tip interacts with the sample, providing the system specific adhesion force (Fad). In this research, it was attached in the functionalized AFM tip the notable encephalitogenic peptides MOG92-106 and MBP85-99 to detect and study the specific antibodies anti-MOG92-106 and anti-MBP85-99 on the sample with AFS technique. Thus, this study was applied for the first time in research with AFS, assisting directly to MS and other demyelinating diseases investigation. (AU) | |
| FAPESP's process: | 14/21530-0 - Imnunonanosensors based on atomic force microscopy for multiple sclerosis studies |
| Grantee: | Pâmela Soto Garcia |
| Support Opportunities: | Scholarships in Brazil - Doctorate |