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Nanoimmunosensors development to neuromyelitis optica survey and detection

Grant number: 15/05283-6
Support Opportunities:Scholarships in Brazil - Doctorate (Direct)
Effective date (Start): July 01, 2015
Effective date (End): June 30, 2019
Field of knowledge:Interdisciplinary Subjects
Principal Investigator:Fabio de Lima Leite
Grantee:Ariana de Souza Moraes
Host Institution: Centro de Ciências e Tecnologias para a Sustentabilidade (CCTS). Universidade Federal de São Carlos (UFSCAR). Sorocaba , SP, Brazil


The Neuromyelitis Optica (NMO) is a disease triggered through recognition of the Aquaporin 4 [Homo sapiens] (AQP4), water channels protein expressed in astrocyte foot process, by the anti-Aquaporin 4 antibody (anti-AQP4). The NMO has clinical picture of optic nerve lesions (neuritis) and transverse myelitis and needs of meticulous studies due its severity. A misdiagnosis of the NMO may result in an elusive diagnosis of Multiple Sclerosis (MS) and to lead the patients to death. Since 2004, anti-AQP4 biomarker is used for differential diagnosis between NMO and MS, through of the Indirect Immunofluorescence (IIF) assay and Enzyme-linked Immunosorbent Assay (ELISA). In this context, this study aims the Immunonanosensor development made with Atomic Force Microscopy tips for NMO biomarker survey and detection. The immunonanosensor will be developed by the chemical modification of the AFM tips and mica muscovite (substrate) nanosurfaces. These nanosurfaces will be functionalized with specifics biomolecules: the AQP4 peptide will be immobilized in the AFM tip and the anti-AQP4 antibody from serological samples of the NMO patients will be immobilized on mica. The interaction between AQP4 peptides versus anti-AQP4 antibodies will be measured by the Atomic Force Spectroscopy (AFS) technique. This research project is innovative due to the development of a novel detection method of the NMO biomarker, using NMO patients, MS patients and healthy patients' serological sample. This study will differentiate serological samples by the presence of anti-AQP4 antibody, besides allowing greater understanding of the NMO mechanism of action. (AU)

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Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MORAES, ARIANA DE SOUZA; BRUM, DORALINA GUIMARAES; MAGALHAES IERICH, JESSICA CRISTIANE; HIGA, AKEMI MARTINS; JABUR ASSIS, AMANDA STEFANIE; MIYAZAKI, CELINA MASSUMI; SHIMIZU, FLAVIO MAKOTO; PERONI, LUIS ANTONIO; MACHINI, M. TERESA; BARREIRA, AMILTON ANTUNES; et al. A highly specific and sensitive nanoimmunosensor for the diagnosis of neuromyelitis optica spectrum disorders. SCIENTIFIC REPORTS, v. 9, . (16/19387-0, 14/50869-6, 13/14262-7, 14/15093-7, 12/50839-4, 14/12082-4, 15/05283-6, 15/06847-0, 15/14360-4)
MAGALHAES IERICH, JESSICA CRISTIANE; BRUM, DORALINA GUIMARAES; MORAES, ARIANA DE SOUZA; HIGA, AKEMI MARTINS; GARCIA, PAMELA SOTO; MIYAZAKI, CELINA MASSUMI; FERREIRA, MARYSTELA; PERONI, LUIS ANTONIO; DE OLIVEIRA, GUEDMILLER SOUZA; FRANCA, EDUARDO DE FARIA; et al. Antibody-mediated biorecognition of myelin oligodendrocyte glycoprotein: computational evidence of demyelination-related epitopes. SCIENTIFIC REPORTS, v. 9, . (13/09746-5, 14/12082-4, 14/15093-7, 14/21530-0, 12/50839-4, 15/05283-6, 14/26369-3, 14/50869-6, 15/06847-0, 16/19387-0)
HIGA, AKEMI M. M.; MORAES, ARIANA S. S.; SHIMIZU, FLAVIO M. G.; BUENO, RAQUEL G. A.; PERONI, LUIS A. T.; STRIXINO, FRANCISCO T.; SOUSA, NISE A. C. G.; DEFFUNE, ELENICE L.; BOVOLATO, ANA LIVIA C.; OLIVEIRA JR, OSVALDO N.; et al. Anti-aquaporin-4 immunoglobulin G colorimetric detection by silver nanoparticles. Nanomedicine-Nanotechnology Biology and Medicine, v. 41, p. 9-pg., . (15/05283-6, 14/50869-6, 15/06847-0, 18/22214-6)
OLIVEIRA, GUEDMILLER S.; IERICH, JESSICA C. M.; MORAES, ARIANA S.; SILVA, GISELA B. R. F.; LIU, YANYUN; NETO, LOURIVAL R. DE S.; FARIA, ROBERTO R.; FRANCA, EDUARDO F.; FREITAS, LUIZ C. G.; BRIGGS, JAMES M.; et al. Immobilization and unbinding investigation of the antigen -antibody complex using theoretical and experimental techniques. JOURNAL OF MOLECULAR GRAPHICS & MODELLING, v. 86, p. 219-227, . (12/50839-4, 15/05283-6, 13/09746-5, 14/21530-0, 14/12082-4, 14/26369-3, 15/06847-0)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
MORAES, Ariana de Souza. Development of the nanoimmunosensor-AQP461-70 for the highly specific and sensitive diagnosis of neuromyelitis optica spectrum disorders. 2019. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Medicina Tropical de São Paulo (IMT) São Paulo.

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