Effect of Poly p 5 allergen from Polybia paulista wasp venom (Hymenoptera, Vespidae) on the modulation of the functional phenotype of murine intraperitoneal macrophages.

Author(s)

Murilo Luiz Bazon

• Document type: Doctoral Thesis
• Press: Rio Claro. 2021-06-07.
• Institution: Universidade Estadual Paulista (Unesp). Instituto de Biociências. Rio Claro
• Defense date: 2021-05-03
• Advisor: Márcia Regina Brochetto Braga; Patrícia Ucelli Simioni

Abstract

The social wasp Polybia paulista (Hymenoptera, Vespidae), abundant in southwestern Brazilian region, is extremely aggressive, being responsible for many medical occurrences. Studies with the allergenic components of this venom have gained increasing interest due to the promising results of its use in laboratory diagnosis, as well as in clinical protocols of specific allergen immunotherapy. One of the most allergenic components found in the P. paulista venom is the Antigen 5 (Poly p 5), which belongs to cysteine-rich secretory proteins (CAP) superfamily. Despite this, its biological function as a component of the Vespidae venom remains unknown. In this context, the possible modulation of the in vitro immune response induced by the allergen Antigen 5 from P. paulista venom, expressed in recombinant form (rPoly p 5) in yeast Komagataella phaffi (Pichia pastoris), on peritoneal macrophages extracted from BALB / c mice was analyzed, activated and not activated with pro-inflammatory stimulus (LPS). For this purpose, were analyzed, in these cells, cell viability changes, expression of the phosphorylated form of NF-κB, nitric oxide (NO) and proinflammatory cytokines, in addition to expression of costimulatory molecules CD80 and CD86. The results indicate that rPoly p 5, at a concentration of 1 μg / mL, does not affect NO production and does not modulate the expression of co-stimulatory molecules CD80 and CD86 in mouse peritoneal macrophages, activated or not with LPS. However, rPoly p 5 induced an increase in IL-1 β (p=0,0463) in macrophages not stimulated with LPS and a reduction in the production of cytokines TNF-α (p= 0,0297) and MCP-1 (p=0,040), when stimulated with LPS. The rPoly p 5 allergen also significantly decreased the in vitro production of the phosphorylated form p65 of NF- κB in macrophages, when compared to the control group (without LPS). The evaluation of the pro or anti-inflammatory potential of the rPoly p 5 allergen, by analyzing the responses of the intraperitoneal macrophages of mice, has not yet led to a clear understanding of the immunomodulatory potential of this protein. However, some findings may indicate an important role for this allergen in the polarization of functional M2 macrophage phenotypes. (AU)

• FAPESP's process: 17/07988-2 - Biological and inflammatory evaluation of the antigen 5 from the venom of Polybia paulista (Hymenoptera, Vespidae) in the recombinant form, on cells of macrophagic lineage
• Grantee: Murilo Luiz Bazon
• Support Opportunities: Scholarships in Brazil - Doctorate