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Biological and inflammatory evaluation of the antigen 5 from the venom of Polybia paulista (Hymenoptera, Vespidae) in the recombinant form, on cells of macrophagic lineage

Grant number: 17/07988-2
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): May 01, 2018
Status:Discontinued
Field of knowledge:Biological Sciences - Biochemistry
Principal Investigator:Márcia Regina Brochetto Braga
Grantee:Murilo Luiz Bazon
Home Institution: Instituto de Biociências (IB). Universidade Estadual Paulista (UNESP). Campus de Rio Claro. Rio Claro , SP, Brazil
Associated scholarship(s):19/02298-3 - Characterization of the immunological potential of the recombinant antigen 5 from Polybia paulista venom, BE.EP.DR

Abstract

In Brazil there are many species of social wasps, which constitute a potential risk to humans. The social wasp Polybia paulista (Hymenoptera, Vespidae) is very aggressive and abundant in the States of São Paulo and South of Minas Gerais, being responsible for many accidents of medical importance. Hymenoptera stings are among the main causes of systemic allergic responses. Whereas anaphylactic shock caused by allergenic venom proteins and associated with immediate hypersensitivity reactions reachs a considerable part of the world population. Interest in the molecular characterization of these allergens has increased in the scientific community due to the promising results obtained in clinical immunological studies, in the therapeutics and prophylaxis of hypersensitivity responses. Many assays seek a better understanding of the allergic processes and can thus improve the diagnostics allowing the identification of epitopes and molecular regions that interact with antibodies. Among the components found in the P. paulista venom is the allergenic protein Antigen 5 (Poly p 5) that has been considered one of the main allergens present in its venom, together with hyaluronidase (Poly p 2) and phospholipase A1 (Poly p 1). In this work, the effects of the ryPoly p 5 protein, corresponding to the recombinant form of the Poly p 5 expressed in Pichia pastoris, will be evaluated on primary cultures of macrophages (obtained from BALB/C lineage mice) by the ability of this allergen to stimulate the release of inflammatory mediators and cytokines related to the inflammatory response. The diversity of venoms components, as well as the scarce knowledge of their immunological potential, as occurred to the Poly p 5 protein, makes relevant to carry out more studies that can elucidate the processes involved both in the sensitization of the stung individual as well as in the search to define better methods of immunotherapy, aiming to the suppression of allergic and inflammatory responses. (AU)