Characterization of the immunological potential of the recombinant antigen 5 from Polybia paulista venom

• Grant number: 19/02298-3
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Effective date (Start): September 09, 2019
• Effective date (End): June 08, 2020
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Márcia Regina Brochetto Braga
• Grantee: Murilo Luiz Bazon
• Supervisor: Simon Blank
• Host Institution: Instituto de Biociências (IB). Universidade Estadual Paulista (UNESP). Campus de Rio Claro. Rio Claro , SP, Brazil
• Research place: Center of Allergy and Environment Munich, Germany
• Associated to the scholarship: 17/07988-2 - Biological and inflammatory evaluation of the antigen 5 from the venom of Polybia paulista (Hymenoptera, Vespidae) in the recombinant form, on cells of macrophagic lineage, BP.DR

Abstract

The social wasp Polybia paulista (Hymenoptera, Vespidae), abundant in southeastern Brazil, is extremely aggressive and responsible for many severe allergic reactions. One of the most allergenic components found in this venom is Antigen 5 (Poly p 5). Although the function of Antigen 5 has not yet been clarified, the molecule is a strong candidate to be used in immunotherapy for patients allergic to social wasp venom. Recently, it was demonstrated by our group that the soluble recombinant form of Poly p 5 (ryPoly p 5) obtained through expression in Komagataella phaffii is allergenic and induces an immune response that is comparable to its native counterpart. As a prerequisite to use ryPoly p 5 for CRD (Component-resolved diagnostics) of P. paulista venom allergy it has to be characterized in detail on a molecular and immunological level. For this characterization, cross-reactivity analyses with crude venom from temperate species are of crucial importance. This is due to the fact that the main diagnostic systems available on the market are based on recombinant allergens of species from the Northern hemisphere. Therefore, in this work we aim at the characterization of the immunological potential of recombinant antigen 5 from Polybia paulista as a prerequisite to use this allergen in the routine diagnosis of allergies to the venom of social Hymenoptera. Moreover, the obtained results could contribute to the understanding of the biological role of the allergen Antigen 5 in Hymenoptera venoms from the region of Europe as well as from the region of South America. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PEREZ-RIVEROL, AMILCAR; MUSACCHIO-LASA, ALEXIS; ROMANI FERNANDES, LUIS GUSTAVO; APARECIDO DOS SANTOS-PINTO, JOSE ROBERTO; ESTEVES, FRANCIELE GREGO; BAZON, MURILO LUIZ; ZOLLNER, RICARDO DE LIMA; PALMA, MARIO SERGIO; BROCHETTO-BRAGA, MARCIA REGINA. Improved production of the recombinant phospholipase A1 from Polybia paulista wasp venom expressed in bacterial cells for use in routine diagnostics. 3 BIOTECH, v. 10, n. 5, APR 27 2020. (14/13936-7, 13/26451-9, 19/02298-3, 17/10373-0, 15/14220-8, 17/07988-2)

BLANK, SIMON; BAZON, MURILO LUIZ; GROSCH, JOHANNES; SCHMIDT-WEBER, CARSTEN B.; BROCHETTO-BRAGA, MARCIA REGINA; BILO, MARIA BEATRICE; JAKOB, THILO. Antigen 5 Allergens of Hymenoptera Venoms and Their Role in Diagnosis and Therapy of Venom Allergy. CURRENT ALLERGY AND ASTHMA REPORTS, v. 20, n. 10, JUL 9 2020. (19/02298-3, 17/07988-2)

BAZON, MURILO LUIZ; FERNANDES, LUIS GUSTAVO ROMANI; ASSUGENI, ISABELA OLIVEIRA SANDRINI; PINTO, LUCAS MACHADO; SIMIONI, PATRICIA UCELLI; ZOLLNER, RICARDO DE LIMA; BRAGA, MARCIA REGINA BROCHETTO. iological and Inflammatory Effects of Antigen 5 from Polybia paulista (Hymenoptera, Vespidae) Venom in Mouse Intraperitoneal Macrophage. TOXINS, v. 13, n. 12, DEC 2021. (17/07988-2, 17/18422-0, 19/02298-3)