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Allergens from venom of the social wasp Polybia paulista (Hymenoptera, Vespidae): recombinant expression in yeast for improved diagnosis and specific immunotherapy to Hymenoptera venom allergy

Grant number: 14/13936-7
Support Opportunities:Regular Research Grants
Duration: February 01, 2015 - July 31, 2017
Field of knowledge:Biological Sciences - Biochemistry - Molecular Biology
Principal Investigator:Márcia Regina Brochetto Braga
Grantee:Márcia Regina Brochetto Braga
Host Institution: Instituto de Biociências (IB). Universidade Estadual Paulista (UNESP). Campus de Rio Claro. Rio Claro , SP, Brazil
Associated researchers:Ricardo de Lima Zollner

Abstract

The social wasp Polybia paulista (Hymenoptera, Vespidae) is quite abundant and endemic in the states of São Paulo and southern Minas Gerais. It has urban habits and is very aggressive, causing many significant accidents of medical perspective. To date, there are no allergenic extracts or components available in our country to diagnosis and treatment of allergic patients to insect venom despite the great number of existing species. Through biochemical, immunological analysis and of IgE recognition in the serum of allergic patients to venom of this wasp, we aimed to perform a comparative analysis of the immunogenic potential of the recombinant allergens (phospholipase A, antigen 5 and hyaluronidase) expressed in Pichia pastoris, in comparison with the venom allergenic native proteins. Our results will contribute to advances in the treatment of specific immunotherapy (IT) and to develop diagnostic kits for allergies caused by the venom of P. paulista and of other closely related species. Taking in account that these are the first recombinant venom allergens from a Brazilian endemic wasp species, this comes to fill a shortage in Brazil since today our patients are treated with wasp venom extracts from temperate countries. (AU)

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VEICULO: TITULO (DATA)

Scientific publications (9)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PEREZ-RIVEROL, AMILCAR; CAMPOS PEREIRA, FRANCO DANI; MUSACCHIO LASA, ALEXIS; ROMANI FERNANDES, LUIS GUSTAVO; APARECIDO DOS SANTOS-PINTO, JOSE ROBERTO; JUSTO-JACOMINI, DEBORA LAIS; DE AZEVEDO, GABRIEL OLIVEIRA; BAZON, MURILO LUIZ; PALMA, MARIO SERGIO; ZOLLNER, RICARDO DE LIMA; et al. Molecular cloning, expression and IgE-immunoreactivity of phospholipase Al, a major allergen from Polybia paulista (Hymenoptera: Vespidae) venom. Toxicon, v. 124, p. 44-52, . (14/13936-7)
PEREZ-RIVEROL, AMILCAR; APARECIDO DOS SANTOS-PINTO, JOSE ROBERTO; MUSACCHIO LASA, ALEXIS; PALMA, MARIO SERGIO; BROCHETTO-BRAGA, MARCIA REGINA. Wasp venomic: Unravelling the toxins arsenal of Polybia paulista venom and its potential pharmaceutical applications. JOURNAL OF PROTEOMICS, v. 161, p. 88-103, . (13/26451-9, 14/13936-7, 11/51684-1)
PEREZ-RIVEROL, AMILCAR; MIEHE, MICHAELA; JABS, FREDERIC; SEISMMAN, HENNING; ROMANI FERNANDES, LUIS GUSTAVO; ZOLLNER, RICARDO DE LIMA; JAKOB, THILO; BROCHETTO BRAGA, MARCIA R.; SPILLNER, EDZARD. Venoms of Neotropical wasps lack cross-reactive carbohydrate determinants enabling reliable protein-based specific IgE determination. Journal of Allergy and Clinical Immunology, v. 141, n. 5, p. 1917+, . (14/13936-7)
ROMANI FERNANDES, LUIS GUSTAVO; PEREZ-RIVEROL, AMILCAR; BAZON, MURILO LUIZ; ABRAM, DEBORA MOITINHO; BROCHETTO-BRAGA, MARCIA REGINA; ZOLLNER, RICARDO DE LIMA. Functional Profile of Antigen Specific CD4(+)T Cells in the Immune Response to Phospholipase A1 Allergen fromPolybia paulistaVenom. TOXINS, v. 12, n. 6, . (14/13936-7, 17/22405-3, 18/24834-1, 19/00729-7)
PEREZ-RIVEROL, AMILCAR; MUSACCHIO-LASA, ALEXIS; ROMANI FERNANDES, LUIS GUSTAVO; APARECIDO DOS SANTOS-PINTO, JOSE ROBERTO; ESTEVES, FRANCIELE GREGO; BAZON, MURILO LUIZ; ZOLLNER, RICARDO DE LIMA; PALMA, MARIO SERGIO; BROCHETTO-BRAGA, MARCIA REGINA. Improved production of the recombinant phospholipase A1 from Polybia paulista wasp venom expressed in bacterial cells for use in routine diagnostics. 3 BIOTECH, v. 10, n. 5, . (14/13936-7, 13/26451-9, 19/02298-3, 17/10373-0, 15/14220-8, 17/07988-2)
PEREZ-RIVEROL, AMILCAR; JUSTO-JACOMINI, DEBORA LAIS; ZOLLNER, RICARDO DE LIMA; BROCHETTO-BRAGA, MARCIA REGINA. Facing Hymenoptera Venom Allergy: From Natural to Recombinant Allergens. TOXINS, v. 7, n. 7, p. 2551-2570, . (14/13936-7)
BAZON, MURILO LUIZ; PEREZ-RIVEROL, AMILCAR; APARECIDO DOS SANTOS-PINTO, JOSE ROBERTO; ROMANI FERNANDES, LUIS GUSTAVO; MUSACCHIO LASA, ALEXIS; JUSTO-JACOMINI, DEBORA LAIS; PALMA, MARIO SERGIO; ZOLLNER, RICARDO DE LIMA; BROCHETTO-BRAGA, MARCIA REGINA. Heterologous Expression, Purification and Immunoreactivity of the Antigen 5 from Polybia paulista Wasp Venom. TOXINS, v. 9, n. 9, . (14/13936-7, 13/26451-9)
BAZON, MURILO LUIZ; SILVEIRA, LAIS HELENA; SIMIONI, PATRICIA UCELLI; BROCHETTO-BRAGA, MARCIA REGINA. Current Advances in Immunological Studies on the Vespidae Venom Antigen 5: Therapeutic and Prophylaxis to Hypersensitivity Responses. TOXINS, v. 10, n. 8, . (17/18422-0, 14/13936-7)
PEREZ-RIVEROL, AMILCAR; ROMANI FERNANDES, LUIS GUSTAVO; MUSACCHIO LASA, ALEXIS; APARECIDO DOS SANTOS-PINTO, JOSE ROBERTO; ABRAM, DEBORA MOITINHO; IZUKA MORAES, GABRIEL HIDEKI; JABS, FREDERIC; MIEHE, MICHAELA; SEISMMAN, HENNING; PALMA, MARIO SERGIO; et al. Phospholipase A1-based cross-reactivity among venoms of clinically relevant Hymenoptera from Neotropical and temperate regions. Molecular Immunology, v. 93, p. 87-93, . (13/26451-9, 14/13936-7, 11/51684-1)

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