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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Functional Profile of Antigen Specific CD4(+)T Cells in the Immune Response to Phospholipase A1 Allergen fromPolybia paulistaVenom

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Author(s):
Romani Fernandes, Luis Gustavo [1] ; Perez-Riverol, Amilcar [2] ; Bazon, Murilo Luiz [3] ; Abram, Debora Moitinho [1] ; Brochetto-Braga, Marcia Regina [3, 4] ; Zollner, Ricardo de Lima [1]
Total Authors: 6
Affiliation:
[1] Univ Estadual Campinas, Med Sch, Lab Translat Immunol, FCM, UNICAMP, BR-13083888 Campinas - Brazil
[2] Sao Paulo State Univ, Ctr Study Social Insects, Inst Biosci Rio Claro, Dept Gen & Appl Biol, BR-13500 Rio Claro - Brazil
[3] Sao Paulo State Univ, Lab Arthropods Mol Biol LBMA IBRC UNESP, BR-13506900 Rio Claro - Brazil
[4] Sao Paulo State Univ UNESP, Venoms & Venomous Anim Studies Ctr CEVAP, BR-18610307 Botucatu, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: TOXINS; v. 12, n. 6 JUN 2020.
Web of Science Citations: 1
Abstract

Insect venom can cause systemic allergic reactions, including anaphylaxis. Improvements in diagnosis and venom immunotherapy (VIT) are based on a better understanding of an immunological response triggered by venom allergens. Previously, we demonstrated that the recombinant phospholipase A1 (rPoly p 1) fromPolybia paulistawasp venom induces specific IgE and IgG antibodies in sensitized mice, which recognized the native allergen. Here, we addressed the T cell immune response of rPoly p 1-sensitized BALB/c mice. Cultures of splenocytes were stimulated withPolybia paulistavenom extract and the proliferation of CD8(+)and CD4(+)T cells and the frequency of T regulatory cells (Tregs) populations were assessed by flow cytometry. Cytokines were quantified in cell culture supernatants in ELISA assays. The in vitro stimulation of T cells from sensitized mice induces a significant proliferation of CD4(+)T cells, but not of CD8(+)T cells. The cytokine pattern showed a high concentration of IFN-gamma and IL-6, and no significant differences to IL-4, IL-1 beta and TGF-beta 1 production. In addition, the rPoly p 1 group showed a pronounced expansion of CD4(+)CD25(+)FoxP3(+)and CD4(+)CD25(-)FoxP3(+)Tregs. rPoly p 1 sensitization induces a Th1/Treg profile in CD4(+)T cell subset, suggesting its potential use in wasp venom immunotherapy. (AU)

FAPESP's process: 14/13936-7 - Allergens from venom of the social wasp Polybia paulista (Hymenoptera, Vespidae): recombinant expression in yeast for improved diagnosis and specific immunotherapy to Hymenoptera venom allergy
Grantee:Márcia Regina Brochetto Braga
Support Opportunities: Regular Research Grants
FAPESP's process: 17/22405-3 - IDENTIFICATION AND SYNTHESIS OF PEPTIDES CORRESPONDING TO B-CELL LINEAR EPITOPES IN ALLERGENS FROM VENOM OF SOCIAL HYMENOPTERA: DEVELOPMENT OF SUPPLIES FOR DIAGNOSIS AND IMMUNOTHERAPY OF ALLERGY
Grantee:Amilcar Perez Riverol
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 18/24834-1 - Synthetic B-cell linear epitopes of allergens from clinically relevant Hymenoptera for improved molecular diagnosis and immunotherapy of venom allergy
Grantee:Amilcar Perez Riverol
Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
FAPESP's process: 19/00729-7 - Immune response modulation to the antigenic components of the Polybia paulista venom(Hymenoptera, Vepidae) in a murine experimental model of allergy: effects of bystander suppression induced by oral tolerance to protein antigen.
Grantee:Luis Gustavo Romani Fernandes
Support Opportunities: Regular Research Grants