| Full text | |
| Author(s): |
Romani Fernandes, Luis Gustavo
[1]
;
Perez-Riverol, Amilcar
[2]
;
Bazon, Murilo Luiz
[3]
;
Abram, Debora Moitinho
[1]
;
Brochetto-Braga, Marcia Regina
[3, 4]
;
Zollner, Ricardo de Lima
[1]
Total Authors: 6
|
| Affiliation: | [1] Univ Estadual Campinas, Med Sch, Lab Translat Immunol, FCM, UNICAMP, BR-13083888 Campinas - Brazil
[2] Sao Paulo State Univ, Ctr Study Social Insects, Inst Biosci Rio Claro, Dept Gen & Appl Biol, BR-13500 Rio Claro - Brazil
[3] Sao Paulo State Univ, Lab Arthropods Mol Biol LBMA IBRC UNESP, BR-13506900 Rio Claro - Brazil
[4] Sao Paulo State Univ UNESP, Venoms & Venomous Anim Studies Ctr CEVAP, BR-18610307 Botucatu, SP - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | TOXINS; v. 12, n. 6 JUN 2020. |
| Web of Science Citations: | 1 |
| Abstract | |
Insect venom can cause systemic allergic reactions, including anaphylaxis. Improvements in diagnosis and venom immunotherapy (VIT) are based on a better understanding of an immunological response triggered by venom allergens. Previously, we demonstrated that the recombinant phospholipase A1 (rPoly p 1) fromPolybia paulistawasp venom induces specific IgE and IgG antibodies in sensitized mice, which recognized the native allergen. Here, we addressed the T cell immune response of rPoly p 1-sensitized BALB/c mice. Cultures of splenocytes were stimulated withPolybia paulistavenom extract and the proliferation of CD8(+)and CD4(+)T cells and the frequency of T regulatory cells (Tregs) populations were assessed by flow cytometry. Cytokines were quantified in cell culture supernatants in ELISA assays. The in vitro stimulation of T cells from sensitized mice induces a significant proliferation of CD4(+)T cells, but not of CD8(+)T cells. The cytokine pattern showed a high concentration of IFN-gamma and IL-6, and no significant differences to IL-4, IL-1 beta and TGF-beta 1 production. In addition, the rPoly p 1 group showed a pronounced expansion of CD4(+)CD25(+)FoxP3(+)and CD4(+)CD25(-)FoxP3(+)Tregs. rPoly p 1 sensitization induces a Th1/Treg profile in CD4(+)T cell subset, suggesting its potential use in wasp venom immunotherapy. (AU) | |
| FAPESP's process: | 14/13936-7 - Allergens from venom of the social wasp Polybia paulista (Hymenoptera, Vespidae): recombinant expression in yeast for improved diagnosis and specific immunotherapy to Hymenoptera venom allergy |
| Grantee: | Márcia Regina Brochetto Braga |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 17/22405-3 - IDENTIFICATION AND SYNTHESIS OF PEPTIDES CORRESPONDING TO B-CELL LINEAR EPITOPES IN ALLERGENS FROM VENOM OF SOCIAL HYMENOPTERA: DEVELOPMENT OF SUPPLIES FOR DIAGNOSIS AND IMMUNOTHERAPY OF ALLERGY |
| Grantee: | Amilcar Perez Riverol |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 18/24834-1 - Synthetic B-cell linear epitopes of allergens from clinically relevant Hymenoptera for improved molecular diagnosis and immunotherapy of venom allergy |
| Grantee: | Amilcar Perez Riverol |
| Support Opportunities: | Scholarships abroad - Research Internship - Post-doctor |
| FAPESP's process: | 19/00729-7 - Immune response modulation to the antigenic components of the Polybia paulista venom(Hymenoptera, Vepidae) in a murine experimental model of allergy: effects of bystander suppression induced by oral tolerance to protein antigen. |
| Grantee: | Luis Gustavo Romani Fernandes |
| Support Opportunities: | Regular Research Grants |