Development of biocatalisators from plant waste for application in biorremediation and biodiesel

Author(s)

Clarissa Hamaio Okino Delgado

• Document type: Doctoral Thesis
• Press: Botucatu. 2017-08-29.
• Institution: Universidade Estadual Paulista (Unesp). Instituto de Biociências. Botucatu
• Defense date: 2017-06-23
• Advisor: Luciana Francisco Fleuri; Márcia Ortiz Mayo Marques

Abstract

Brazilian agroindustrial processing has sought the maximum conversion of waste into byproducts. In this context, the fruit processing stands out due to the rich composition of the parts initially considered as residues, which may include high value biomolecules such as enzymes and bioactive compounds. Among the enzymes are lipases that catalyze reactions of hydrolysis and synthesis of glycerides and which have promising market growth expectations due to the current demand for biofuels and bioremediation. In this context, the present work evaluated the wastes from processing from orange to juice and found that these can be used as direct sources of lipases. Orange lipases have high activity in hydrolysis and synthesis reactions, ranging from acid to alkaline pH (6.0 to 8.0), thermostability up to 50 ° C and affinity for substrates of different chain sizes (p-nPP, P-NPL and PNPB); this can be concentrated by ammonium sulfate precipitation and immobilized by adsorption; and have a synergistic effect with fungal lipases and potential for application in bioremediation of cooking oil residues. The results obtained are presented in five chapters. The first one consists of the bibliographical review on the potential of the residues generated in the processing of Brazilian fruits as sources of lipases and the inclusion of this process in the concept of biorefineries; The second describes the obtaining and biochemical characterization of lipases obtained from orange residues of different varieties; Chapter 3 presents the optimization of the activity of these lipases by immobilization and synergism with fungal lipases; Chapter 4 includes the evaluation of orange lipases in bioremediation of cooking oil; And chapter 5 describes the study on the financial and biochemical viability of biodiesel production by chemical and enzymatic catalysis from different plant oils. (AU)

• FAPESP's process: 14/10962-7 - Development of biocatalysts from plant wastes for application in bioremediation and biodiesel production
• Grantee: Clarissa Hamaio Okino Delgado
• Support Opportunities: Scholarships in Brazil - Doctorate