Proteinogram and serum immunoglobulin G concentration in foals from birth to thirty days of life, treated with plasma

Author(s)

Campo Amor Vieira da Cunha Neto

• Document type: Master's Dissertation
• Press: Botucatu. 2016-07-20.
• Institution: Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária e Zootecnia. Botucatu
• Defense date: 2016-07-04
• Advisor: Alexandre Secorun Borges; João Pessoa Araújo Júnior

Abstract

The aim of this study was to evaluate the protein concentrations and serum IgG in foals birth to thirty days, before and after suckling colostrum and be treated with plasma and evaluate the quality of colostrum and plasma used. Twenty foals and their mothers were used and four donor animals (plasma). colts blood samples were collected in five times (M1 = zero, M2 = ten M3 = 24, M4 = 48 hours and M5 = 30 days), blood and colostrum progenitor mares at foaling. Plasma Serum samples were evaluated for total protein and albumin concentrations and refraction by colorimetric technique. Serum protein was obtained by electrophoresis on an agarose gel, and the total globulin has been estimated through mathematical calculation. The mean ± standard deviation of the serum IgG concentration of foals group of the M1 at M5, measured by ELISA, was of 15±8 mg/dL, 2,408±608 mg/dL, 2,282±783 mg/dL, 2,364±784 mg/dl and 1.414±586 mg/dL, respectively. Serum IgG and colostral (ELISA) were 1.746±505 mg/dL and 7.714±2619 mg/dl, respectively. There was a strong correlation between the results obtained by both quantitation of IgG tests. However, the IgG concentration determined by SRID in all samples were statistically higher than those obtained by ELISA. (AU)

• FAPESP's process: 14/13328-7 - Evaluate the kinetics of immunoglobulins in foals Thoroughbred birth at thirty days of age treated with hyperimmune plasma
• Grantee: Campo Amor Vieira da Cunha Neto
• Support Opportunities: Scholarships in Brazil - Master