The mutagenic potential of an environmental pollutant 2-phenilbenzotriazole-9 non chlorinated (non-cl pbta-9) in vivo

Author(s)

Amanda Rodrigues A. R. Tanamachi

• Document type: Master's Dissertation
• Press: Botucatu. 2020-05-04.
• Institution: Universidade Estadual Paulista (Unesp). Instituto de Biociências. Botucatu
• Defense date: 2020-03-02
• Advisor: Daisy Maria Fávero Salvadori; Fábio Henrique Fernandes

Abstract

The constantly expanding industrial sector has been generating residues with high toxic potential to the environment. The textile industry has a heavily impact, polluting the hydrosphere due to chemical processes used. Literature shows that even after effluent treatment, toxic compounds are still present in the wastewater and in rivers. Moreover, the water decontamination can make some dyes compounds even more toxics. Currently, environmental pollution caused by azo dyes, their byproducts and intermediates, has been widely investigated. In this sense, the non-chlorinated 2-phenilbenzotriazole 9 (non-Cl PBTA 9) has received attention because it is derived from the dye Disperse Violet 93, which is detected in high quantity in surface waters under influence of textile activities. Thus, the aim of this study was to investigate the toxicogenomic potential of acute exposure to the non-chlorinated PBTA-9 in mice. The three doses tested (5, 50 and 500 μg/kg body weight) of the compound were orally (gavage) administered to the animals. Micronucleus frequency in bone marrow cells, primary DNA damage in blood, liver and colon cells, and gene (TP53, CYP1A1, NAT2 and CDKN1A) expression profiling in liver cells were analyzed. The results showed that the non-Cl PBTA 9 was genotoxic in blood and liver cells at the highest dose (500 μg/kg b.w.) and at doses of 5, 50 and 500 μg/kg b.w. in colon cells. Mutagenic effect in bone marrow cells was observed at 5 and 50 μg/kg b.w.. No histological alterations and gene expression modulation were detected in liver. In conclusion, the data showed that the non-Cl PBTA 9, byproduct of DV93, even at low concentrations, presented a genotoxic potential, suggesting that water contamination by this compound may be harmful to the environment and human health. (AU)

• FAPESP's process: 18/04105-5 - Toxicogenomic potential of environmental contaminant 2-phenylbenzotriazole 9 (PBTA-9) in vivo
• Grantee: Amanda Rodrigues Tanamachi
• Support Opportunities: Scholarships in Brazil - Master