Effect of solutions and gels containing a sugarcane-derived cystatin on enamel and dentin erosive wear in vitro

Author(s)

Lethycia Almeida Santos

• Document type: Master's Dissertation
• Press: Bauru.
• Institution: Universidade de São Paulo (USP). Faculdade de Odontologia de Bauru (FOB/SDB)
• Defense date: 2021-03-19

Examining board members

Marilia Afonso Rabelo Buzalaf; Alessandra Buhler Borges; Heitor Marques Honório; Juliano Pelim Pessan

• Advisor: Marilia Afonso Rabelo Buzalaf

Abstract

The protective effect of solutions and gels containing a sugarcane-derived cystatin (CaneCPI-5) on erosive wear of enamel and dentin was evaluated in vitro. Bovine enamel and dentin specimens were divided into two groups (n = 60 and 68 / group for enamel and dentin, respectively) that were treated with solutions or 3% chitosan-based gels containing 0.1 or 0.25 mg/ml CaneCPI-5. The positive controls for solutions and gels were Elmex Erosion Protection solution (Colgate) and 12,300 ppm F 3% chitosan-based gel (as NaF), respectively. Negative controls were deionized water and 3% chitosan-based placebo gel, respectively. Stimulated saliva was collected from 3 donors to form the acquired pellicle (for 2 h) on the specimens. The specimens were submitted to an erosive pH cycling protocol 4 times/day for 7 days (0.1% citric acid pH 2.5/90s, artificial saliva/2h, artificial saliva overnight). The solutions and gels were applied during pH cycling, 2 times/day for 1 min and 4 min, respectively, after the first and last erosive challenges. Enamel and dentin wear (m) were assessed by contact profilometry. Data were analyzed by 3-way ANOVA and Tukey´s test (p <0.05). All the treatments significantly reduced enamel and dentin loss in comparison with placebo. For enamel, both CaneCPI-5 concentrations were similar to the positive controls, while for dentin, only 0.25 mg/ml CaneCPI-5 was similar to the positive controls. Regarding the vehicles, only the 0.1 mg/ml gel performed worse than the positive control. In conclusion, CaneCPI-5 reduces enamel and dentin wear to a similar extent as the fluoridated vehicles. Moreover, dentin seems to require higher CaneCPI-5 concentrations, especially in the case of gels. Solutions or chitosan-based gels containing CaneCPI-5 might be a new approach to protect against erosive tooth wear. (AU)

• FAPESP's process: 18/22729-6 - Effect of solution and gel containing a new cistatin derived from sugar cane in erosion of enamel and dentin in vitro
• Grantee: Lethycia Almeida Santos Azuma
• Support Opportunities: Scholarships in Brazil - Master