Full text
| |
| Author(s): |
Luiz Henrique Moraes Caetano de Camargo
Total Authors: 1
|
| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Faculdade de Medicina (FM/SBD) |
| Defense date: | 2021-10-05 |
| Examining board members: |
José Angelo Lauletta Lindoso;
André Gustavo Tempone Cardoso;
Martha Simões Ribeiro;
Juliana Ferreira Strixino
|
| Advisor: | José Angelo Lauletta Lindoso |
| Abstract | |
Introduction: Leishmaniasis can be present under different clinical manifestations and caused by different species of Leishmania. Photodynamic therapy (PDT) has demonstrated promising effects on leishmaniasis treatment, but it is necessary to investigate the mechanisms of action of this therapy on different Leishmania species. Objective: To analyze the effects of PDT with methylene blue and red LED (light-emitting diode) on L. amazonensis and L. braziliensis promastigotes and amastigotes. Methods: L. amazonensis and L. braziliensis were used to evaluate the effects of PDT in promastigotes. J774 macrophages were infected with both species and submitted to PDT. The cell viability was analyzed with MTT and flow cytometry. Gene expression of pro and anti-inflammatory cytokines were analyzed with qPCR. Results: Promastigotes and amastigotes of L. amazonensis and L. braziliensis demonstrated decrease in cell viability when submitted to PDT, but on flow cytometry analysis this difference was not significant. With PDT, the gene expression of pro-inflammatory cytokines were increased and regarding anti-inflammatory cytokines, PDT has shown an increase on gene expression. Conclusion: PDT caused parasite inactivation, with best results in the highest concentration and power density independent of the species (AU) | |
| FAPESP's process: | 18/12426-6 - Effects of photodynamic therapy on the cell viability of Leishmania braziliensis and Leishmania amazonensis |
| Grantee: | Luiz Henrique Moraes Caetano de Camargo |
| Support Opportunities: | Scholarships in Brazil - Master |